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The Escherichia coli recA gene increases UV-induced mitotic gene conversion in Saccharomyces cerevisiae

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Abstract

The effect of the Escherichia coli RecA protein on mitotic recombination in the diploid D7 strain of Saccharomyces cerevisiae damaged by UV radiation was investigated. The D7 strain was transformed by two modified versions of the pNF2 plasmid: one, containing the ADH-1 promoter, and the other containing the recA gene tandemly arranged behind the ADH-1 promoter region. Immunological analysis proved the presence of the 38-kDa RecA protein in D7/pNF2ADHrecA transformants. We observed a positive effect of recA gene expression on mitotic gene conversion, mainly at higher doses of UV radiation. The results indicate that a RecA-like activity could participate in steps preceeding mitotic conversion events in yeast.

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References

  • Aboussekhra A, Chanet R, Adjiri A, Fabre F (1992) Mol Cell Biol 12:3224–3234

    Google Scholar 

  • Andrade HHR, Marques EK, Schenberg ACG, Henriques JAP (1989) Mol Gen Genet 217:419–426

    Google Scholar 

  • Aquilera A, Klein HL (1989) Genetics 123:683–694

    Google Scholar 

  • Bishop DK, Park D, Xu L, Kleckner N (1992) Cell 59:439–456

    Google Scholar 

  • Brozmanová J, Černáková L, Vlčková V, Duraj J, Fridrichová I (1991) Mol Gen Genet 227:473–480

    Google Scholar 

  • Cox MM (1991) Mol Microbiol 5:1295–1299

    Google Scholar 

  • Henriques JAP, Vicente JE, da Silva KVCL, Schenberg ACG (1989) Mutat Res 218:111–124

    Google Scholar 

  • Howard-Flanders P, West SC, Stasiak A (1984) Nature 309:215–220

    Google Scholar 

  • Kans JA, Mortimer RK (1991) Gene 105:139–140

    Google Scholar 

  • Malone RE, Esposito RE (1980) Proc Natl Acad Sci USA 77:503–507

    Google Scholar 

  • Müller B, Burdet I, West SC (1992) EMBO J 11:2685–2693

    Google Scholar 

  • Saeki T, Machida I, Nakai S (1980) Mutat Res 73:251–265

    Google Scholar 

  • Schiestl A, Prakash S (1990) Mol Cell Biol 10:2485–2491

    Google Scholar 

  • Shinohara A, Ogawa H, Ogawa T (1992) Cell 69:457–470

    Google Scholar 

  • Webster TD, Dickson RC (1983) Gene 26:243–252

    Google Scholar 

  • Zimmermann FK, Kern R, Rasenberger H (1975) Mutat Res 31:381–388

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Genetics, Comenius University, Faculty of Science, Mlynská dolina B1, 842 15, Bratislava, Slovakia

    Viera Vlčková

  2. Cancer Research Institute, Slovak Academy of Science, 812 32, Bratislava, Slovakia

    Luba Černáková, Eva Farkašová & Jela Brozmanová

Authors
  1. Viera Vlčková
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  2. Luba Černáková
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  3. Eva Farkašová
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  4. Jela Brozmanová
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Communicated by R. J. Schweyen

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Vlčková, V., Černáková, L., Farkašová, E. et al. The Escherichia coli recA gene increases UV-induced mitotic gene conversion in Saccharomyces cerevisiae . Curr Genet 25, 472–474 (1994). https://doi.org/10.1007/BF00351789

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  • DOI: https://doi.org/10.1007/BF00351789

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