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Isolation and structure of an acetolactate synthase gene from Schizosaccharomyces pombe and complementation of the ilv2 mutation in Saccharomyces cerevisiae

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Abstract

A gene encoding a functional acetolactate synthase (ALS) subunit has been isolated from the fission yeast Schizosaccharomyces pombe, and has been structurally and genetically characterized. The approximate 5-kbp cloned DNA segment was found to contain a 2007-bp open reading frame capable of encoding a 669 aminoacid polypeptide which exhibited 57.1% similarity to the corresponding ALS subunit from Saccharomyces cerevisiae. The putative ilv1 isolated from S. pombe was shown to encode a functional subunit of acetolactate synthase by complementation of an S. cerevisiae strain deleted for the ILV2 locus.

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Author notes

  1. Susan A. Nadin-Davis

    Present address: Agriculture Canada, Animal Disease Research Institute, NEPEAN, 300, Station H, P.O. Box 11, K2H-8P9, Nepean, ON, Canada

Authors and Affiliations

  1. Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N-0W9, Saskatoon, SK, Canada

    Faouzi Bekkaoui & William L. Crosby

  2. Institute for Biological Sciences, National Research Council of Canada, 100 Sussex Drive, K1A-0R6, Ottawa, ON, Canada

    Susan A. Nadin-Davis

Authors
  1. Faouzi Bekkaoui
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  2. Susan A. Nadin-Davis
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  3. William L. Crosby
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Communicated by B. A. Kunz

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Bekkaoui, F., Nadin-Davis, S.A. & Crosby, W.L. Isolation and structure of an acetolactate synthase gene from Schizosaccharomyces pombe and complementation of the ilv2 mutation in Saccharomyces cerevisiae . Curr Genet 24, 544–547 (1993). https://doi.org/10.1007/BF00351720

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  • DOI: https://doi.org/10.1007/BF00351720

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