Abstract
Dose-response relationship in eyestalk-less Palaemon adspersus was studied with a reference standard preparation of red-pigment-concentrating hormone from eyestalks of Pandalus borealis. The response, which is an expression for the degree of concentration of pigment in the small red integumentary chromatophores during a 60 min period following injection of hormone, was recorded using the Hogben and Slome system for quantitating chromatophore dispersion. The responses were normally distributed, with equal variances at different doses and the mean responses showed a linear regression on log-dose. The index of precision (λ) was about 0.20. Two munits (about 2×10-12 g) of pure red-pigment-concentrating hormone were sufficient to cause a significant response. One mg of lyophilized eyestalks of Pandalus borealis contains about 0.9 units of red-pigment-concentrating hormone. The red-pigment-concentrating hormone activity of eyestalks from Palaemon adspersus shows the same dose-response relationship as the hormone standard and amounts to about 2.9 units/mg of lyophilized eyestalks.
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Communicated by E. Steemann Nielsen, Copenhagen
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Fernlund, P. Chromactivating hormones of Pandalus borealis . Marine Biol. 2, 13–18 (1968). https://doi.org/10.1007/BF00351632
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DOI: https://doi.org/10.1007/BF00351632