Abstract
Crude molluscan lipase was prepared by homogenizing in tris buffer either fresh or lyophilized crystalline styles of Spisula solidissima. Both preparations showed specific activities less than 2% of standard hog pancreatic lipase, and pH and temperature optima of 8.0 and 20°C, respectively. When compared with mammalian hog pancreatic lipase, the clam lipase was found to be the more versatile enzyme on primary wax esters as well as methyl esters. Neither lipase was capable of hydrolyzing the oleate or palmitate esters of the secondary alcohols of glycerol, cholesterol or isopropyl alcohol; however, both showed activity on the oleate ester of isobutyl alcohol, a primary alcohol. S. solidissima lipase, hog pancreatic lipase, and a lipase isolated from the pancreas of the skate Raja erinacea, all demonstrated an inability to hydrolyze the methyl esters of eicosatetraenoic, eicosapentaenoic and docosahexaenoic acids. The lipase-resistant polyunsaturated fatty acid esters are discussed in terms of their role in the lipid biochemistry of marine organisms.
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Literature cited
Arnott, H. J., J. A. C. Nicol and C. W. Querfeld: Reflecting spheres in the eyes of the weakfishes (Sciaenidea). Nature, Lond. 233, 130–133 (1971).
Bottino, N. R., G. A. Vandenburg and R. Reiser: Resistance of certain long-chain polyunsaturated fatty acids of marine oils to pancreatic lipase hydrolysis. Lipids 2, 489–493 (1967).
Brockerhoff, H.: Digestion of fat by cod. J. Fish. Res. Bd Can. 23, 1835–1839 (1966).
—: Substrate specificity of pancreatic lipase: Influence of the structure of fatty acids on the reactivity of esters. Biochim. biophys. Acta 212, 92–101 (1970).
— and R. J. Hoyle: Hydrolysis of triglycerides by the pancreatic lipase of a skate. Biochim. biophys. Acta 98, 435–436 (1965).
— and P. C. Huang: Incorporation of fatty acids of marine origin into triglycerides and phospholipids of mammals. Biochim. biophys. Acta 144, 541–548 (1967a).
—, J. E. Stowart and W. Tacreiter: Digestion of triglycerides by lobster. Can. J. Biochem. 45, 421–422 (1967b).
George, W. C.: The digestion and absorption of fat in lamellibranchs. Biol. Bull. mar. biol. Lab., Woods Hole 102, 118–127 (1952).
Herb, S. F. and V. G. Martin: How good are analyses of oils by GLC. J. Am. Oil Chem. Soc. 47, 415–421 (1970).
Hozumi, M.: On the lipolytic enzyme in the crystalline style of the clam, Venerupis philippinarum Adams et Reeve. Sci. Rep. Tokyo Kyoiku Daig. (Section B) 9, 37–55 (1959).
—: Studies on the lipase in the digestive diverticula of the clam, Venerupis philippinarum Adams et Reeve. Sci. Rep. Tokyo Kyoiku Daig. (Section B) 10, 155–186 (1961).
International Union of Biochemistry 1964 Recommendations: Enzyme nomenclature, 128 pp. New York: Elsevier Publishing Co. 1965.
Isihara, Y.: Studies on whale lipase. I. Lipase of the pancreas of little finner. Bull. Fac. Fish. Hokkaido Univ. 11–12, 23–28 (1960–1962).
IUPAC-IUB Commission on Biochemical Nomenclature (CBN): The nomenclature of lipids. Eur. J. Biochem. 2, 127–131 (1967).
Jensen, R. G.: Lipolytic enzymes. In: Progress in the chemistry of fats and other lipids, 11. pp 347–394. Ed. by R. T. Holman. New York: Pergamon Press 1971
Kleiman, R., F. R. Earl and I. A. Wolff: The trans-3-enoic acids of Grindelia oxylepis seed oil (abstract). J. Am. Oil Chem. Soc. 42, p. 147A (1965)
Litchfield, C. and A. M. Greenberg: Comparative lipid patterns in the head fats of dolphins, porpoises and toothed whales (abstract). J. Am. Oil Chem. Soc. 49, p. 307A (1972).
Litwack, G.: Chemical determinations of tyrosine and protein by the Folin-Ciocalteu reaction. In: Experimental biochemistry, pp 145–150. New York: John Wiley & Sons Inc. 1960.
Lowry, O. H., N. J. Roseborough and A. L. Farr: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–270 (1951).
Luddy, F. E., R. A. Barford, S. F. Herb, P. Magidman and R. W. Riemenschneider: Pancreatic lipase hydrolysis of triglycerides by a semimicro technique. J. Am. Oil Chem. Soc. 41, 693–696 (1964).
Mattson, F. H. and R. A. Volpenhein: Behavior of lipids at interfaces and in biological membranes: enzymatic hydrolysis at an oil-water interface. J. Am. Oil. Chem. Soc. 43, 286–289 (1966).
McNair, H. M. and E. J. Bonelli: Basic gas chromatography, 5th ed. 306 pp. Walnut Creek, California: Varian Aerograph 1969.
Metcalfe, L. D., A. A. Schmitz and J. R. Pelka: Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Analyt. Chem. 38, 514–515 (1966).
Patton, J. S.: Studies on the digestive lipase of the surf clam, Spisula solidissima, 102 pp. M.S. Thesis, University of Rhode Island, Kingston, R.I. 1972.
Quinn, J. G.: Large scale preparation of highly purified mixed acid triglycerides, 53 pp. Ph. D. Thesis, University of Connecticut, Storrs, Conn. 1967.
—, J. Sampugna and R. G. Jensen: Synthesis of 100-gram quantities of highyl purified mixed acid triglycerides. J. Am. Oil Chem. Soc. 44, 439–442 (1967).
Ropes, J. W., J. L. Chamberlin and A. S. Merrill: Surf clam fishery. In: Encyclopedia of marine resources, pp 119–125. Ed. by F. E. Firth. New York: Van Nostrand Reinhold Co. 1969.
Wills, E. D.: Lipases. Adv. Lipid Res. 3, 197–240 (1965).
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Communicated by J. Bunt, Miami
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Patton, J.S., Quinn, J.G. Studies on the digestive lipase of the surf clam Spisula solidissima . Marine Biology 21, 59–69 (1973). https://doi.org/10.1007/BF00351192
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DOI: https://doi.org/10.1007/BF00351192