Abstract
Transgenic mice generated with different DNA sequences were surveyed for possible homozygous mutant phenotypes. We found an embryonic lethal mutation in the transgenic mouse strain (MT-MYC12.4) containing the human c-myc gene. Embryos homozygous for the transgene die shortly after implantation. The strain MT-MYC12.4 carries approximately 50 tandem copies of the recombinant plasmid sequence. The 3′ flanking sequence has been cloned and analyzed. It contains a unique sequence that has been conserved during evolution and maps to Chromosome (Chr) 9. This mutant has been designated Tg 9 (HSA-MYC).
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Tutois, S., Salaun, J., Mattei, MG. et al. Tg (9 HSA-MYC), a homozygous lethal insertion in the mouse. Mammalian Genome 1, 184–190 (1991). https://doi.org/10.1007/BF00351065
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DOI: https://doi.org/10.1007/BF00351065