Abstract
In analysis of the repeats from the mink X Chromosome (Chr), we have identified a B2-like repetitive sequence of 195 base pairs (bp) flanked by short direct repeats of 14 bp. It contains regions homologous to the split intragenic RNA polymerase III promoter and a 3′ A-rich region followed by an oligo(dA) sequence. A feature of the repeat is the presence of a perfect polypyrimidine tract 22 bp in length absent from the known Alu- and Alu-like sequences. Alignment of the mink B2-like sequence and mouse B2-consensus sequence allowed us to estimate their similarity as 55%. The repeat is present in 1–2×105 copies per mink genome and 2–4×103 copies per X Chr. In situ hybridization analysis demonstrated a similar distribution pattern of the B2-like repeat along the length of all the mink chromosomes including the X. We also observed the presence of mink B2-like hybridizable sequence in the genomes of other Carnivora species.
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The nucleotide sequence data reported in this paper have been submitted to EMBL Data Library and have been assigned the accession number X52381 (MVB2RPT).
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Lavrentieva, M.V., Rivkin, M.I., Shilov, A.G. et al. B2-like repetitive sequence from the X Chromosome of the American mink (Mustela vison). Mammalian Genome 1, 165–170 (1991). https://doi.org/10.1007/BF00351063
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DOI: https://doi.org/10.1007/BF00351063