Skip to main content
SpringerLink
Log in

Meiotic mapping of murine chromosome 17: The string of loci around l(17)-2Pas

  • Original Contributions
  • Published:
Mammalian Genome Aims and scope Submit manuscript

Abstract

We describe a genetic analysis of l(17)-2Pas, an embryonic lethal mutation on murine chromosome 17. Males transmitted the l(17)-2 allele to only 38% of their offspring, whereas females transmitted this allele at 50%. Two-point crosses revealed tight linkage between l(17)-2 and brachyury (T), and deletion mapping placed l(17)-2 outside of the hairpin-tail deletion (T hp). To map this mutation more precisely, we intercrossed hybrid mice that carry distinct alleles at many classical and DNA loci on chromosome 17 and obtained 172 animals recombinant in the T to H-2 region. Strong positive interference was observed over the 14 cM interval from T to H-2K. Thus, a single recombinant can be informative; one such recombinant places l(17)-2 distal of the molecular marker D17Leh66D. Robust genetic maps can be constructed with multilocus crosses that share anchor loci. DNA markers can be interpolated onto these maps retrospectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Amar, L., Dandolo, C.L., Hanauer, A., Cook, A.R., Arnaud, D., Mandel, J.-L., and Avner, P.: Conservation and reorganization of loci on the mammalian X chromosome: A molecular framework for the identification of homologous subchromosomal regions in man and mouse. Genomics 2: 220–230, 1988.

    Google Scholar 

  • Avner, P., Amar, L., Arnaud, D., Hanauer, A., and Cambrou, J.: Detailed ordering of markers localizing to the Xq26-Xqter region of the human X chromosome by the use of an interspecific Mus spretus mouse cross. Proc Natl Acad Sci USA 84: 1629–1633, 1987.

    Google Scholar 

  • Avner, P., Amar, L., Dandolo, L., and Guénet, J.-L.: Genetic analysis of the mouse using interspecific crosses. Trends Genet 4: 18–23, 1988.

    Google Scholar 

  • Birkenmeier, C.S., McFarland-Starr, E.C., and Barker, J.E.: Chromosomal location of three spectrin genes: relationship to the inherited hemolytic anemias of mouse and man. Proc Natl Acad Sci USA 85: 8121–8125, 1988.

    Google Scholar 

  • Bennett, D., Dunn, L.C., Spiegelman, K., Artzt, K., Cookingham, J., and Schermerhorn, E.: Observations on a set of radiation-induced dominant T-like mutations in the mouse. Genet Res 26: 95–108, 1975.

    Google Scholar 

  • Bhattacharyya, G.K., and Johnson R.A.: Statistical Concepts and Methods, pp. 249, 308–312, John Wiley & Sons, New York, 1977.

    Google Scholar 

  • Bode, V.C.: Ethylnitrosourea mutagenesis and the isolation of mutant alleles for specific genes located in the T region of mouse chromosome 17. Genetics 108: 457–470, 1984.

    Google Scholar 

  • Búcan, M., Herrmann, B.G., Frischauf, a.-M., Bautch, V.L., bode, V., Silver, L.M., Martin, G.R., and Lehrach, H.: Deletion and duplication of DNA sequences is associated with the embryonic lethal phenotype of the t9 complementation group of the mouse t complex. Genes Dev 1: 376–385, 1987.

    Google Scholar 

  • Buchberg, A.M., Jenkins, N.A., and Copeland, N.G.: Localization of the murine macrophage colony-stimulating factor to chromosome 3 using interspecific backcross analysis. Genomics 5: 363–367, 1989.

    Google Scholar 

  • Buchberg, A.M., Brownell, E., Nagata, S., Jenkins, N.A., and Copeland, N.G.: A comprehensive genetic map of murine chromosome 11 reveals extensive linkage conservation between mouse and human. Genetics 122: 153–161, 1989.

    Google Scholar 

  • Bullard, D.C., and Schimenti, J.C.: Molecular cloning and genetic mapping of the t complex responder candidate gene family. Genetics (in press).

  • Burke, D.T., Carle, G.F., and Olson, M.V.: Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806–812, 1987.

    Google Scholar 

  • Ceci, J.D., Siracusa, L.D., Jenkins, N.A., and Copeland, N.G.: A molecular genetic linkage map of mouse chromosome 4 including the localization of several proto-oncogenes. Genomics 5: 699–709, 1989.

    Google Scholar 

  • Ceci, J.D., Justice, M.J., Lock, L.F., Jenkins, N.A., and Copeland, N.G.: An interspecific backcross linkage map of mouse chromosome 8. Genomics 6: 72–79, 1990.

    Google Scholar 

  • Cheng, S.V., Martin, G.R., Nadeau, J.H., Haines, J.L., Bucan, M., Kozak, C.A., MacDonald, M.E., Lockyer, J.L., Ledley, F.D., Woo, S.L.C., Lehrach, H., Gilliam, T.C., and Gusella, J.F.: Synteny on mouse chromosome 5 of homologues for human DNA loci linked to the Huntington disease gene. Genomics 4: 419–426, 1989.

    Google Scholar 

  • Coulson, A., Waterston, R., Kliff, J., Sulston, J., and Kohara, Y.: Genome linking with yeast artificial chromosomes. Nature 335: 184–186, 1988.

    Google Scholar 

  • Crow, J.F.: Mapping functions. Genetics 125: 669–671, 1990.

    Google Scholar 

  • Dandoy, F., Demaeyer-Guignard, J., and Demaeyer, E.: Linkage analysis of the murine mos proto-oncogene on chromosome 4. Genomics 4: 546–551, 1989.

    Google Scholar 

  • Davisson, M.T., and Roderick, T.H.: Linkage Map. In M.F. Lyon and A.G. Searle (eds.): Genetic Variants and Strains of the Laboratory Mouse (Second Edition), pp. 416–427, Oxford University Press, New York, 1989.

    Google Scholar 

  • Dove, W.F.: Molecular genetics of Mus musculus: Point mutagenesis and millimorgans. Genetics 118: 5–8, 1987.

    Google Scholar 

  • Eckert, K.A., Ingle, C.A., Klinedinst, D.K., and Drinkwater, N.R.: Molecular analysis of mutations induced in human cells by N-ethyl-N-nitrosourea. Mol Carcin 1: 50–56, 1988.

    Google Scholar 

  • Ehling, U.H., and Neuhäuser-Klaus, A: Dose effect relationships of germ-cell mutations in mice. In Y. Tazima (ed.): Problems of Threshold in Chemical Mutagenesis, pp. 15–25, The Environmental Mutagen Society of Japan, 1984.

  • Eppig, J.T.: List of mouse DNA clones and probes. Mouse News Lett 83: 73–117, 1989.

    Google Scholar 

  • Feinberg, A.P., and Vogelstein, B.: A technique for radiolabelling endonuclease fragments to high specific activity. Anal Biochem 137: 266–267, 1984.

    Google Scholar 

  • Glaser, T., Matthews, K.E., Hudson, J.W., Seth, P., Housman, D.E., and Crerar, M.M.: Localization of the muscle, liver, and brain glycogen phosphorylase genes on linkage maps of mouse chromosomes 19, 12, and 2 respectively. Genomics 5: 510–521, 1989.

    Google Scholar 

  • Hammer, M.F., Schimenti, J., and Silver, L.M.: Evolution of mouse chromosome 17 and the origin of inversions associated with t haplotypes. Proc Natl Acad Sci USA 86: 3261–3265, 1989.

    Google Scholar 

  • Helentjaris, T., and Gesteland, R.: Evaluation of random cDNA clones as probes for human restriction fragment polymorphisms. J Mol Appl Genet 2: 237–247, 1983.

    Google Scholar 

  • Herrmann, B.G., Barlow, D.P., and Lehrach, H.: A large inverted duplication allows homologous recombination between chromosomes heterozygous for the proximal t complex inversion. Cell 48: 813–825, 1987.

    Google Scholar 

  • Herrmann, B., Bucan, M., Mains, P.E., Frischauf, A.-M., Silver, L.M., and Lehrach, H.: Genetic analysis of the proximal portion of the mouse t complex: evidence for a second inversion within t haplotypes. Cell 44: 469–476, 1986.

    Google Scholar 

  • Herrmann, B.G., Labeit, S., Poustka, A.-M., King, T.R., and Lehrach, H.: Cloning of the T gene required in mesoderm formation in mouse. Nature 343: 617–622, 1990.

    Google Scholar 

  • Hitotsumachi, S., Carpenter, D.A., and Russell, W.L.: Doserepetition increases the mutagenic effectiveness of N-ethyl-N-nitrosourea in mouse spermatogonia. Proc Natl Acad Sci USA 82: 6619–6621, 1985.

    Google Scholar 

  • Johnson, D.R.: Further observations on the hairpin-tail (T hp) mutation in the mouse. Genet Res 24: 207–213, 1975.

    Google Scholar 

  • Johnson, F.M., and Lewis, S.E.: Electrophoretically detected germinal mutations induced in the mouse by ethylnitrosourea. Proc Natl Acad Sci USA 78: 3138–3141, 1981.

    Google Scholar 

  • Justice, M.J., Silan, C.M., Ceci, J.D., Buchberg, A.M., Copeland, N.G., and Jenkins, N.A.: A molecular genetic linkage map of mouse chromosome 13 anchored by the beige (bg) and satin (sa) loci. Genomics 6: 341–351, 1990.

    Google Scholar 

  • King, T.R., and Dove, W.F.: Pleiotropic action of the murine quaking locus: structure of the qk v allele. Mammalian Genome 1: 000-000, 1990.

    Google Scholar 

  • King, T.R., Dove, W.F., Herrmann, B.G., Moser, A.R., and Shedlovsky, A.: Mapping to molecular resolution in the T to H-2 region of the mouse genome with a nested set of meiotic recombinants. Proc Natl Acad Sci USA 86: 222–226, 1989.

    Google Scholar 

  • Kingsley, D.M., Jenkins, N.A., and Copeland, N.G.: A molecular genetic linkage map of mouse chromosome 9 with regional localizations for the Gsta, T3g, Est-1 and Ldlr loci. Genetics 123: 165–172, 1989.

    Google Scholar 

  • Laird, C.D.: Chromatid structure: relationship between DNA content and nucleotide sequence diversity. Chromosoma 32: 378–406, 1971.

    Google Scholar 

  • Lander, E.S., and Green, P.: Construction of multilocus genetic linkage maps in humans. Proc Natl Acad Sci USA 84: 2363–2367, 1987.

    Google Scholar 

  • Lathrop, G.M., and Lalouel, J.-M.: Efficient computations in multilocus linage analysis. Am J Hum Genet 42: 498–505, 1988.

    Google Scholar 

  • Lathrop, G.M., Lalouel, J.-M., Julier, C., and Ott, J.: Strategies for multilocus linkage analysis in humans. Proc Natl Acad Sci USA 81: 3443–3446, 1984.

    Google Scholar 

  • Loveless, A.: Possible relevance of O-6 alkylation of deoxyguanosine to the mutagenicity and carcinogenicity of nitrosamines and nitrosamides. Nature 223: 206–207, 1969.

    Google Scholar 

  • Mann, E.A., Silver, L.M., and Elliott, R.W.: Genetic analysis of a mouse t complex locus that is homologous to a kidney cDNA clone. Genetics 114: 993–1006, 1986.

    Google Scholar 

  • Morton, N.E., Maclean, C.J., Lew, R., and Yee, S.: Multipoint linkage analysis. Am J Hum Genet 38: 868–883, 1986.

    Google Scholar 

  • Moseley, W.S., and Seldin, M.F.: Definition of mouse chromosome 1 and 3 gene linkage groups that are conserved on human chromosome 1: evidence that a conserved linkage group spans the centromere of human chromosome 1. Genomics 5: 899–905, 1989.

    Google Scholar 

  • Mullins, L.J., Grant, S.G., Stephenson, D.A., and Chapman, V.M.: Multilocus molecular mapping of the mouse X chromosome. Genomics 3: 187–194, 1988.

    Google Scholar 

  • Nobile, C., and Romeo, G.: Partial digestion with restriction enzymes of ultraviolet-irradiated human genomic DNA: a method for identifying restriction site polymorphisms. Genomics 3: 272–274, 1988.

    Google Scholar 

  • Peters, J.: Ethylnitrosourea as a mouse mutagen. Trends Genet 1: 5–6, 1985.

    Google Scholar 

  • Poustka, A.-M., and Lehrach, H.: Jumping libraries and linking libraries: The next generation of molecular tools in mammalian genetics. Trends Genet 2: 174–179, 1986.

    Google Scholar 

  • Readhead, C., Popko, B., Takahashi, N., Shine, H.D., Saavedra, R.A., Sidman, R.L., and Hood, L.: Expression of a myelin basic protein gene in transgenic shiverer mice: correction of the dismyelinating phenotype. Cell 48: 703–712, 1987.

    Google Scholar 

  • Rigby, P.W.J., Dieckmann, M., Rhodes, C., and Berg, P.: Labelling deoxyribonucleic acids to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113: 237–251, 1977.

    Google Scholar 

  • Rohme, D., Fox, H., Herrmann, B., Frischauf, A.-M., Edstrom, J.-E., Mains, P., Silver, L.M., and Lehrach, H.: Molecular clones of the mouse t complex derived from microdissected metaphase chromosomes. Cell 36: 783–788, 1984.

    Google Scholar 

  • Russell, W.L.: X-ray-induced mutations in mice. Cold Spring Harbor Symp Quant Biol XVI: 327–336, 1951.

    Google Scholar 

  • Russell, W. L., Hunsicker, P.R., and Maddux, S.C.: Dose repetition greatly increases the mutagenic effectiveness of ENU in mouse spermatogonia: additional data. 5th International Conference on Environmental Mutagens (Abstract), 1989.

  • Russell, W.L., Kelly, E.M., Hunsicker, P.R., Bangham, J.W., Maddux, S.C., and Phipps, E.L.: Specific locus test shows ethylnitrosourea to be the most potent mutagen in the mouse. Proc Natl Acad Sci USA 76: 5818–5819, 1979.

    Google Scholar 

  • Russell, W.L., Hunsicker, P.R., Raymer, G.D., Steele, M.H., Stelzner, K.F., and Thompson, H.M.: Dose response curve for ethylnitrosourea-induced-specific-locus mutations in mouse spermatogonia. Proc Natl Acad Sci USA 79: 3589–3591, 1982.

    Google Scholar 

  • Scherer, G., Bausch, E., Gaa, A., and Von Deimling, O.: Gene mapping on mouse chromosome 8 by interspecific crosses: new data on a linkage group conserved on human chromosome 16q. Genomics 5: 275–282, 1989.

    Google Scholar 

  • Seldin, M.F., Howard, T.A., and D'Eustachio, P.: Comparison of linkage maps of mouse chromosome 12 derived from laboratory strain intraspecific and Mus spretus interspecific backcross. Genomics 5: 24–28, 1989.

    Google Scholar 

  • Seldin, M.F., Morse, H.C. III, Reeves J.P., Scribner, C.L., Leboeuf, R.C., and Steinberg, A.D.: Genetic analysis of autoimmune gld mice. I. Identification of a restriction fragment length polymorphism closely linked to the gld mutation within a conserved linkage group. J Exp Med 167: 688–693, 1988.

    Google Scholar 

  • Shedlovsky, A., Guénet, J.-L., Johnson, L.L., and Dove, W.F.: Induction of recessive lethal mutations in the T/t-H-2 region of the mouse genome by a point mutagen. Genet Res 47: 135–142, 1986.

    Google Scholar 

  • Shedlovsky, A., King, T.R., and Dove, W.F.: Saturation germ line mutagenesis of the murine t region including a lethal allele at the quaking locus. Proc Natl Acad Sci USA 85: 180–184, 1988.

    Google Scholar 

  • Silver, L.M., Artzt, K., and Bennett, D.: A major testicular cell protein specified by a mouse T/t complex gene. Cell 17: 275–284, 1979.

    Google Scholar 

  • Silver, L.M., Lukralle, D., and Garrels, J.I.: T Orl is a novel, variant form of mouse chromosome 17 with a deletion in a partial t haplotype. Nature 301: 422–424, 1983.

    Google Scholar 

  • Siracusa, L.D., Buchberg, A.M., Copeland, N.G., and Jenkins, N.A.:: Recombinant inbred strain and interspecific backcross analysis of molecular markers flanking the murine agouti coat color locus. Genetics 122: 669–679, 1989.

    Google Scholar 

  • Siracusa, L.D., Silan, C.M., Justice, M.J., Mercer, J.A., Bauskin, A.R., Ben-Neriah, Y., Duboule, D., Hastie, N.D., Copeland, N.G., and Jenkins, N.A.: A molecular genetic linkage map of mouse Chromosome 2. Genomics 6: 491–504, 1990.

    Google Scholar 

  • Sturtevant, A.H., and Morgan, T.H.: Reverse mutation of the Bar gene correlated with crossing over. Science 57: 746–747, 1923.

    Google Scholar 

  • Vincek, V., Kawaguchi, H., Mizuno, K., Zaleska-Rutczynska, Z., Kasahara, M., Forejt, J., Figueroa, F., and Klein, J.: Linkage map of mouse chromosome 17: localization of 27 new DNA markers. Genomics 5: 773–786, 1989.

    Google Scholar 

  • Vogel, E., and Natarajan, A.T.: Recessive lethal mutations and translocations in Drosophila. Mutat Res 62: 51–100, 1979.

    Google Scholar 

Download references

Author information

Author notes

  1. Thomas R. King

    Present address: The Jackson Laboratory, 600 Main Street, 04609, Bar Harbor, Maine, USA

Authors and Affiliations

  1. Laboratory of Genetics and McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, 53706, Madison, Wisconsin, USA

    Thomas R. King, William F. Dove & Alexandra Shedlovsky

  2. Génétique des Mammifères, Institut Pasteur, 25 rue du Docteur Roux, 75015, Paris, France

    Jean-Louis Guénet

  3. Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35, D-7400, Tübingen 1, West Germany

    Bernhard G. Herrmann

Authors
  1. Thomas R. King
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. William F. Dove
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jean-Louis Guénet
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bernhard G. Herrmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Alexandra Shedlovsky
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

King, T.R., Dove, W.F., Guénet, JL. et al. Meiotic mapping of murine chromosome 17: The string of loci around l(17)-2Pas . Mammalian Genome 1, 37–46 (1991). https://doi.org/10.1007/BF00350844

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00350844

Keywords

Search

Navigation