Abstract
A particle control system is described for automatic collection of samples, and determination and control of the density of particles in marine suspensions. Densities are determined electronically, allowing colourless microorganisms to be detected. Computer operated magnetic valves allow multiple cultures to be sampled, measured and fed, using compressed air and a vacuum pump. Conditions and variables, determined in the software, enable flexible adjustment to specific culture conditions. The system was applied in the cultivation of the copepod Acartia clausi Giesbrecht, using a 95 μm orifice with a particle size range of 8 to 32 μm. Some results are given of monitoring and controlling the density of heterotrophic flagellates as food in the copepod cultures at different conditions of temperature and autotrophic food.
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Flynn, K. J., Fielder, J. (1989). Changes in intracellular and extracellular amino acids during the predation on the chlorophyte Dunaliella primolecta by the heterotrophic dinoflagellate Oxyrrhis marina and the use of the glutamine/glutamate ratio as an indicator of nutrient status in mixed populations. Mar. Ecol. Prog. Ser. 53: 117–127
Gallager, S. M., Mann, R. (1980). An apparatus for the measurement of grazing activity of filter feeders at constant food concentrations. Mar. Biol. Lett. 1: 341–349
Grecay, P. A. (1989). An apparatus for monitoring and controlling turbidity in biological experiments. Mar. Biol. 103: 421–426
Haupt, K. (1979). A simple device to control concentrations of food algae in feeding experiments with filter feeding organisms. Veröff. Inst. Meeresforsch. Bremerh. 17: 241–244
Hornbach, D. J., Wilcox, T., Layne, J., Powers, L., Davis, T. (1991). A method for assessing clearance rates in suspension-feeding organisms by means of a fiber-optic colorimeter. Can. J. Zool. 69: 2703–2706
Jackson, C. J., Pendry, R. C., Rothlisberg, P. C. (1992). The Larvatron: a computer-controlled apparatus for rearing planktonic animals under experimental conditions. Mar. Ecol. Prog. Ser. 86: 189–199
Janssen, H. H., Dreyer, H. P. (1981). A simple and cheap apparatus for the controlled feeding of suspension feeders. Mar. Biol. 62: 313–315
Klein Breteler, W. C. M., Gonzalez, S. R. (1986). Culture and development of Temora longicornis (Copepoda, Calanoida) at different conditions of temperature and food. Syllogeus (Nat. Mus. Can.) 58: 71–84
Klein Breteler, W. C. M., Schogt, N., Gonzalez, S. R. (1990). On the role of food quality in grazing and development of life stages, and genetic change of body size during cultivation of pelagic copepods. J. exp. mar. Biol. Ecol. 135: 177–189
Pierce, R. W., Turner, J. T. (1992). Ecology of planktonic ciliates in marine food webs. Rev. aquat. Sciences 6: 139–181
Rissgård, H. V., Mohlenberg, F. (1979). An improved automatic recording apparatus for determining the filtration rate of Mytilus edulis as a function of size and algal concentration. Mar. Biol. 52: 61–67
Sinnett, J. C., Davis, W. R. (1983). A programmable tuzbidostate for suspended particles in laboratory aquaria. J. exp. mar. Biol. Ecol. 73: 167–174
Stoecker, D. K., McDowell Capuzzo, J. (1990). Predation on Protozoa: its importance to zooplankton. J. Plankton Res. 12: 891–908
Vijverberg, J. (1989). Culture techniques for studies on the growth, development and reproduction of copepods and cladocerans under laboratory and in situ conditions: a review. Freswat. Biol 21: 317–373
Winter, J. E. (1973). The filtration rate of Mytilus edulis and its dependence on algal concentration, measured by a continuous automatic recording apparatus. Mar. Biol. 22: 317–328
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Communicated by O. Kinne, Oldendorf/Luhe
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Klein Breteler, W.C.M., Lann, M. An apparatus for automatic counting and controlling density of pelagic food particles in cultures of marine organisms. Marine Biology 116, 169–174 (1993). https://doi.org/10.1007/BF00350744
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DOI: https://doi.org/10.1007/BF00350744