Skip to main content
Log in

RASA contains a polymorphic microsatellite and maps to bovine syntenic group U22 on Chromosome 7q2.4-qter

  • Original Contributions
  • Published:
Mammalian Genome Aims and scope Submit manuscript

Abstract

The bovine gene for the p21ras protein activator (RASA) includes in its 5′ untranslated region a (TG)n repeat. Analysis of this (TG)n repeat by PCR amplification of genomic DNA revealed a four-allele polymorphism. A cDNA probe was used to assign RASA to the region 2.4-qter of bovine Chromosome (Chr) 7 by in situ hybridization. PCR analysis of a panel of somatic hybrid lines allowed the assignment of RASA to the unassigned syntenic group 22 (U22) and thus localizes U22 on Chr 7.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Beckmann, J.S. and Soller, M.: Toward a unified approach to genetic mapping of eukaryotes based on sequence tagged microsatellite sites. Biotechnology 8: 930–932, 1990.

    Google Scholar 

  • Botstein, D., White, R.L., Skolnick, M. and Davis, R.W.: Construction of a genetic linkage map in man using restriction fragment length polymorphism. Am J Hum Genet 32: 314–331, 1980.

    Google Scholar 

  • Chevalet, C. and Corpet, F.: Statistical decision rules concerning synteny or independence between markers. Cytogenet Cell Genet 43: 132–139, 1986.

    Google Scholar 

  • Feinberg, A.P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13, 1983.

    Google Scholar 

  • Fries, R.: The bovine gene map. Unpublished data.

  • Fries, R., Hediger, R., and Stranzinger, G.: Tentative chromosomal localization of the bovine major histocompatibility complex by in situ hybridization. Anim Genet 17: 287–294, 1986.

    Google Scholar 

  • Fries, R., Eggen, A., and Stranzinger, G.: The bovine genome contains polymorphic microsatellites. Genomics 8: 403–406, 1990.

    Google Scholar 

  • Fries, R., Solinas, S., and Gunawardana, A.: Chromosomal assignment of marker loci to monitor marker spacing. In L.B. Schoock, H.A. Lewin, and D.G. McLaren (eds), Gene Mapping: Strategies, Techniques and Applications. pp. 159–176, Marcel Dekker Inc. New York, 1991.

    Google Scholar 

  • Georges, M., Gunawardana, A., Threadgill, D.W., Lathrop, M., Olsacker, I., Mishra, A., Sargeant, L.L., Schoerberlein, A., Steele, M.R., Terry, C., Threadgill, D.S., Zhao, X., Holm, T., Fries, R., and Womack, J.E.: Characterization of a set of variable number of tandem repeat markers conserved in bovidae. Genomics 11: 24–32, 1991.

    Google Scholar 

  • Hsieh, C.L., Vogel, U.S., Dixon, R.A.F., and Francke, U.: Chromosome localization and cDNA sequence of murine and human genes for ras p21 GTPase activating Protein (GAP). Somat Cell Mol Genet 15: 579–590, 1989.

    Google Scholar 

  • ISCNDA, 1989. International System for Cytogenetic Nomenclature of Domestic Animals. Cytogenet Cell Genet 53: 65–79, 1990.

    Google Scholar 

  • Lowy, D.R., Zhang, K., Declue, J.E., and Willumsen, B.M.: Regulation of p21ras activity. Trends Genet 7: 346–351, 1991.

    Google Scholar 

  • Moore, S.S., Sargeant, L.L., King, T.J., Mattik, J.S., Georges, M., and Hetzel, D.J.S.: The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related species. Genomics 10: 654–660, 1991.

    Google Scholar 

  • O'Brien, S.J.: Mammalian genome mapping: lessons and prospects. Curr Opinion Genet Dev 1: 105–111, 1991.

    Google Scholar 

  • O'Brien, S.J. and Marshall Graves, J.A.: Report of the committee on comparative gene mapping. Cytogenet Cell Genet 58: 1124–1151, 1991.

    Google Scholar 

  • Sanger, F., Nicklen, S., and Coulson, A.R.: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467, 1977.

    Google Scholar 

  • Steffen, P., Eggen, A., and Fries, R.: Method for isolating microsatellites by the consequent use of PCR. Cytogenet Cell Genet 58: 2153–2154, 1991.

    Google Scholar 

  • Sundaresan, S., Yang-Feng, T.L., and Francke, U.: Genes for HMG-CoA reductase and serotonin 1a receptor are on mouse chromosome 13. Somat Cell Mol Genetic 15: 465–469, 1989.

    Google Scholar 

  • Vogel, U.S., Dixon, R.A.F., Schaber, M.D., Diehl, R.E., Marshall, M.S., Scolnick, E.M., Sigal, I.S., and Gibbs, J.B.: Cloning of bovine GAP and its interaction with oncogenic ras p21. Nature 335: 90–93, 1988.

    Google Scholar 

  • White, R., Leppert, M., Bishop, D.T., Barker, D., Berkowitz, J., Brown, C., Callahan, P., Holm, T., and Jerominski, L.: Construction of linkage maps with DNA markers for human chromosomes. Nature 313: 101–105, 1985.

    Google Scholar 

  • Womack, J.E.: Genetic engineering in agriculture: animal genetics and development. Trends Genet 3: 65–68, 1987.

    Google Scholar 

  • Womack, J.E.: Strategies and technologies for comparative gene mapping. In L.B. Schoock, H.A. Lewin, and D.G. McLaren (eds), Gene Mapping: Strategies, Techniques and Applications, pp. 159–176, Marcel Dekker Inc., New York, 1991.

    Google Scholar 

  • Womack, J.E. and Moll, Y.D.: Gene map of the cow: conservation of linkage with mouse and man. J Hered 77: 2–7, 1986.

    Google Scholar 

  • Womack, J.E., Dietz, A.B., Gallagher, D.S., Li, L., Zhang, N., Neibergs, H.L., Moll, Y.D., and Ryan, A.M.: Assignment of 47 additional comparative anchor loci to the bovine synteny map. Cytogenet Cell Genet 58: 2132, 1991.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Eggen, A., Solinas-Toldo, S., Dietz, A.B. et al. RASA contains a polymorphic microsatellite and maps to bovine syntenic group U22 on Chromosome 7q2.4-qter. Mammalian Genome 3, 559–563 (1992). https://doi.org/10.1007/BF00350621

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00350621

Keywords

Navigation