Paired H-2-specific monoclonal antibodies react differently to red cells and lymphocytes

Abstract

Two hybrid clones from a fusion of C57BL/6 anti-DBA/2 spleen cells and the myeloma line Sp2/0 secrete antibodies reactive with a product of the murine major histocompatibility complex (MHC). The two antibodies are provisionally designated 513.11 and 513.29. Both react in rabbit-complementmediated cytotoxicity with spleen cells of H-2 ^d, H-2 ^f, H-2 ^r, and H-2 ^p strains. In addition, both antibodies hemagglutinate red blood cells from these strains. S13.11 is also cytotoxic for H-2 ^a, H-2 ^k, H-2 ^u, and H-2 ^v spleen cells but does not hemagglutinate red blood cells from mice bearing these haplotypes. With the exception of H-2 ^v, this strain pattern mimics the public specificity H-2.8. Quantitative absorption of S13.11 shows that H-2 ^d cells are twice as efficient as H-2 ^k cells in their ability to remove the 513.11 antibody. 513.29 reacts weakly in cytotoxicity with H-2 ^kv spleen cells and does not react with cells from H-2 ^u or H-2 ^v. Blocking studies indicate that 513.11 and S13.29 react with the same or a closely related molecule on the cell surface.