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KLH-specific, I-E/C-restricted clones of proliferating T lymphocytes

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Abstract

The recognition of keyhole limpet hemocyanin by a substantial proportion of proliferating clones of murine T lymphocytes was found to be restricted by the I-E/Ck molecule, which is a combinatorial product of genes located in the I-A (Ae) and I-EIC (Eα) subregions of the murine major histocompatibility complex. The respective roles of the Ae (polymorphic) and Eα (oligomorphic) gene products in the expression of the structures which are used as restriction elements by these T-cell clones was analyzed by mating parental strains unable to present the antigen and bearing selected Ae and Eα alleles. Efficient complementation for antigen presentation was found to require the expression by accessory cells of the Aek-gene product, whereas all Eα allelic molecules were functionally equivalent. These results (a) indicate that the immunoregulatory role of I-region gene products, initially described for molecules selected for their limited number of antigenic epitopes, also applies to complex multiepitopic antigens; (b) illustrate the advantage which results from the diversity of the la molecules expressed by accessory cells for the development of potent immune responses; and (c) suggest that a correlation might exist between the degree of polymorphism of a given family of H-2 allelic molecules and their ability to be used as restriction elements for antigen recognition by T lymphocytes.

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Abbreviations

FCS:

fetal calf serum

Hepes:

N-2-hydroxypiperazine-N′-2 ethanesulfonic acid; in polypeptides

G:

glutamate

L:

lysine

Ø:

phenylalanine

KLH:

keyhole limpet hemocyanin

m. Ab.:

monoclonal antibodies

T cells:

thymus-derived cells

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Authors and Affiliations

  1. Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Case 906, 13288, Marseille Cedex 9, France

    Patrice C. Dubreuil, Danielle H. Caillol & Francois A. Lemonnier

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  1. Patrice C. Dubreuil
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  2. Danielle H. Caillol
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  3. Francois A. Lemonnier
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Dubreuil, P.C., Caillol, D.H. & Lemonnier, F.A. KLH-specific, I-E/C-restricted clones of proliferating T lymphocytes. Immunogenetics 14, 469–479 (1981). https://doi.org/10.1007/BF00350119

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  • DOI: https://doi.org/10.1007/BF00350119

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