Marine Biology

, Volume 116, Issue 2, pp 231–239 | Cite as

Differential sensitivity of marine infaunal amphipods to tributyltin

  • J. P. Meador
  • U. Varanasi
  • C. A. Krone
Article

Abstract

Three species of infaunal gammaridean amphipods, Rhepoxynius abronius (Phoxocephalidae), Eohaustorius washingtonianus, and E. estuarius (Haustoriidae) were tested in a water-only system to assess their sensitivity to tributyltin (TBT) without the influence of factors that could affect bioavailability. When mortality (LC50) was the endpoint, the results indicated that R. abronius was ≈20 times more tolerant to tributyltin than either haustoriid species; however, when mortality plus reburial behavior (EC50) was assessed, the difference was only about 10 times. The bioconcentration factor (BCF) was also consistently lower in R. abronius (11.1 to 16.5 times) than in the haustoriids; however, when the LD50 was calculated, the concentration in the tissues associated with 50% mortality for each species was not significantly different. The large disparity in species' response is attributed to reduced uptake and a potentially greater ability to metabolize this compound by R. abronius. An analysis of TBT uptake confirmed that R. abronius was able to accumulate less TBT and hence maintain a low body burden for a given water concentration. The results of a separate uptake study were used to formulate a hypothesis for observed differences in reburial behavior. Because the rate of TBT uptake was lower in R. abronius, we propose that the slower rise in toxicant body burden allowed for a gradual response in this species which included a sublethal effect (non reburial), compared to a rapid rise in the body burden for E. estuarius which caused the response to quickly proceed from no effect to death.

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Literature cited

  1. APHA (1985). Standard methods for the examination of water and wastewater. 16th ed. American Public Health Association, American Water Works Association, and American Public Health Association, Washington, D.C.Google Scholar
  2. ASTM (1990). Standard guide for conducting 10-day static sediment toxicity tests with marine and estuarine amphipods. E 1367-90. Annual Book of ASATM Standards. American Society for Testing and Materials, Philadelphia, PennsylvaniaGoogle Scholar
  3. Barnard, J. L., Drummond, M. M. (1982). Gammaridean Amphipoda of Australia. Part V. Superfamily Haustorioidea. Smithsonian Institution Press, Washington, D.C. (Smithson. Contr. Zool. 360: 1–148)Google Scholar
  4. Baron, M. G. (1990). Bioconcentration. Envir. Sci. Technol. 24: 1612–1618Google Scholar
  5. Bosworth, W. S. Jr. (1976). Biology of the genus Eohaustorius (Amphipoda: Haustoriidae) on the Oregon coast. Ph. D. dissertation. Oregon State University, CorvallisGoogle Scholar
  6. Buhler, D. R., Williams, D. E. (1989). Enzymes involved in metabolism of PAH by fishes and other aquatic animals: oxidative enzymes (or phase I enzymes) In: Varanasi, U. (ed.) Metabolism of polycyclic aromatic hydrocarbons in the aquatic environment. CRC Press, Boca Raton, Florida, p. 151–184Google Scholar
  7. Bushong, S. J., Hall, L. W. Jr., Hall, W. S., Johnson, W. E., Herman, R. L. (1988). Acute toxicity of tributyltin to selected Chesapeake Bay fish and invertebrates. Wat. Res. 22: 1027–1032Google Scholar
  8. Cardwell R. D., Meador, J. P. (1989). Tributyltin in the environment: an overview and key issues. In: Ocean's 89 Proceedings, September 1989. Marine Technological Society, Seattle, Washington, p. 537–544Google Scholar
  9. Carter, R. J., Turoczy, N. J., Bond, A. M. (1989). Container adsorption of tributyltin (TBT) compounds: implication for environmental analysis. Envir. Sci. Technol. 23: 615–617Google Scholar
  10. DeWitt, T. H. Swartz, R. C., Lamberson, J. O. (1989). Measuring the acute toxicity of estuarine sediments. Envir. Toxic. Chem. 8: 1035–1048Google Scholar
  11. Fitzwater, S. E., Knauer, G. A., Martin, J. H. (1982). Metal contamination and its effect on primary production measurements. Limnol. Oceanogr. 27: 544–551Google Scholar
  12. Herbes, S. E., Allen, C. P. (1983). Lipid quantification of freshwater invertebrates: method modification for microquantitation. Can. J. Fish. aquat. Sciences 40: 1315–1317Google Scholar
  13. Krahn, M. M., Moore, L. K., Bogar, R. G., Wigren, C. A., Chan, S.-L., Brown, D. W. (1988). High-performance liquid chromatographic method for isolating organic contaminants from tissue and sediment extracts. J. Chromatogr. 437: 161–175Google Scholar
  14. Krone, C. A., Brown, D. W., Burrows, D. G., Bogar, R. G., Chan, S.-L., Varanasi, U. (1989). A method of analysis of butyltin species and measurement of butyltins in sediment and English sole livers from Puget Sound. Mar. envirl Res. 27: 1–18Google Scholar
  15. Landrum, P. F. (1988). Toxicokinetics of organic xenobiotics in the amphipod, Pontoporeia hoyi: role of physiological and environmental variables. Aquat. Toxic. 12: 245–271Google Scholar
  16. Laughlin, R. B. Jr., French, W. (1989). Population-related toxicity responses to two butyltin compounds by zoeae of the mud crab Rhithropanopeus harrisii. Mar. Biol. 102: 397–401Google Scholar
  17. Laughlin, R. B., Guard, H. E., Coleman, W. M. III. (1986). Tributyltin in seawater: speciation and octanol-water partition coefficient. Envir. Sci. Technol. 20: 201–204Google Scholar
  18. Laughlin, R. B. Jr., Johannesen, R. B., French, W., Guard, H., Brinckman, F. E. (1985). Structure-activity relationships for organotin compounds. Envir. Toxic. Chem. 4: 343–351Google Scholar
  19. Laughlin, R., Nordlund, K., Linden, O. (1984). Long-term effects of tributyltin on the Baltic amphipod, Gammarus oceanicus. Mar. envirl Res. 12: 243–271Google Scholar
  20. LeBlanc, G. A. (1984). Interspecies relationships in acute toxicity of chemicals to aquatic organisms. Envir. Toxic. Chem. 3: 47–60Google Scholar
  21. Lee, R. F. (1985). Metabolism of tributyltin oxide by crabs, oysters, and fish. Mar. envirl Res. 17: 145–148Google Scholar
  22. Mackay, D. (1982). Correlation of bioconcentration factors. Envir. Sci. Technol. 16: 274–278Google Scholar
  23. McCarty, L. S. (1986). The relationship between aquatic toxicity QSARs and bioconcentration for some organic chemicals. Envir. Toxic. Chem. 5: 1071–1080Google Scholar
  24. McCarty, L. S. (1991a). Toxicant body residues: implication for aquatic bioassays with some organic chemicals. In: Mayes, M. A., Barron, M. G. (eds.) Aquatic toxicology and risk assessment. Vol. 14. American Society for Testing and Materials, Philadelphia, Pa., p. 183–192 (ASTM Ref. 1124)Google Scholar
  25. McCarty, L. S. (1991b). Interpreting aquatic toxicity QSARs: the significance of toxicant body residues at the pharmacological endpoint. Sci. total Envir. 109/110: 515–525Google Scholar
  26. Meador, J. P. (1993). The effect of laboratory holding on the toxicity response of marine infaunal amphipods to cadmium and tributyltin. (in preparation)Google Scholar
  27. Meador, J. P., Salazar, M. H., U'Ren, S. C. (1984). A flow-through bioassay system for the evaluation of organotin compounds. Wat. Res. 18: 647–650Google Scholar
  28. Neely, W. B. (1979). Estimating rate constants for the uptake and clearance of chemicals by fish. Envir. Sci. Technol. 13: 1506–1510Google Scholar
  29. Oakden, J. M. (1984). Feeding and substrate preference in five species of phoxocephalid amphipods from central California. J. Crustaccan Biol. 4: 233–247Google Scholar
  30. Okamoto, K. (1991). Biological reference materials for metal speciation: NIES fish tissue reference material for organotin compounds. In: Subramanian, K. S., Iyengar, G. V., Okamoto, K. (eds.) Biological trace element research: multidisciplinary perspectives. American Chemical Society, Washington, D.C., p. 257–264 (ACS Symp. Ser. No. 445)Google Scholar
  31. Oliver, B. G., Niimi, A. J. (1985). Bioconcentration factors of some halogenated organics for rainbow trout: limitations in their use for prediction of environmental residues. Envir. Sci. Technol. 19: 842–849Google Scholar
  32. Oliver, J. S., Oakden J. M., Slattery, P. N. (1982). Phoxocephalid amphipod crustaceans as predators on larvae and juveniles in marine soft-bottom communities. Mar. Ecol. Prog. Ser. 7: 179–184Google Scholar
  33. Pastorok, R. A., Becker, D. S. (1990). Comparative sensitivity of sediment toxicity bioassays at three Superfund sites in Puget Sound. In: Landis, W. G., van der Schalie (eds.) Aquatic toxicology and risk assessment. Vol. 13. American Society for Testing and Materials. Philadelphia, Pa., p. 123–139 (ASTM Ref. STP 1096)Google Scholar
  34. Reichert, W. L., Eberhart, B.-T. L., Varanasi, U. (1985). Exposure of two species of deposit feeding amphipods to sediment-associated [3H]benzo[a]pyrene: uptake, metabolism, and covalent binding to tissue macromolecules. Aquat. Toxic. 6: 45–56Google Scholar
  35. Salazar, M. H. (1989). Mortality, growth, and bioaccumulation in mussels exposed to TBT: differences between the laboratory and the field. In: Oceans '89. Marine Technological Society, Seattle, Washington, D.C., p. 530–536Google Scholar
  36. Slattery, P. N. (1985). Life histories of infaunal amphipods from subtidal sands of Monterey Bay, California. J. Crustacean Biol. 5: 635–649Google Scholar
  37. Southworth, G. R., Keffer, C. C., Beauchamp, J. J. (1980). Potential and realized bioconcentration: a comparison of observed and predicted bioconcentration of azaarenes in the fathead minnow (Pimephales promelas). Envir. Sci. Technol. 14: 1529–1531Google Scholar
  38. Southworth, G. R., Keffer, C. C., Beauchamp, J. J. (1981). The accumulation and disposition of benz(a)acridine in the fathead minnow (Pimephales promelas). Archs envir. Contam. Toxic. 10: 561–569Google Scholar
  39. Stephan, C. E. (1977). Methods for calculating an LC50. In: Mayer, F. L., Hamelink, J. L. (eds.) Aquatic toxicology and hazard evaluation. American Society for Testing and Materials, Philadelphia, Pa., p. 65–84 (Ref. ASTM STP 634)Google Scholar
  40. Stephan, C. E., Mount, D. I., Hansen, D. J., Gentile, J. H., Chapman, G. A., Brungs, W. A. (1985). Guidelines for deriving numerical national water quality criteria for the protection of aquatic organisms and their uses. U.S. Environmental Protection Agency, Corvallis, OregonGoogle Scholar
  41. Suter, G. W. II, Vaughan, D. S., Gardner, R. H. (1983). Risk assessment by analysis of extrapolation error: a demonstration for effects of pollutants on fish. Envir. Toxic. Chem. 2: 369–378Google Scholar
  42. Tas, J. W., Seinen, W., Opperhuizen, A. (1991). Lethal body burden of triphenyltin chloride in fish: preliminary results. Comp. Biochem. Physiol. 100C: 59–60Google Scholar
  43. Tsuda, T., Aoki, S., Kojima, M., Harada, H. (1990a). Differences between freshwater and seawater-acclimated guppies in the accumulation and excretion of tri-n-butyltin chloride and triphenyltin chloride. Wat. Res. 24: 1373–1376Google Scholar
  44. Tsuda, T., Aoki, S., Kojima, M., Harada, H. (1990b). The influence of pH on the accumulation of tri-n-butyltin chloride and triphenyltin chloride in carp. Comp. Biochem. Physiol. 95C: 151–153Google Scholar
  45. Tsuda, T., Aoki, S., Kojima, M., Harada, H. (1991). Accumulation of tri-n-butyltin chloride and triphenyltin chloride by oral and via gill intake of goldfish (Crassius auratus). Comp. Biochem. Physiol. 99C: 69–72Google Scholar
  46. Tsuda, T., Aoki, S., Kojima, M., Harada, H. (1992). Accumulation and excretion of tri-n-butyltin chloride and triphenyltin chloride by willow shiner. Comp. Biochem. Physiol. 101C: 67–70Google Scholar
  47. Van Hoogen, G., Opperhuizen, A. (1988). Toxicokinetics of chlorobenzenes in fish. Envir. Toxic. Chem. 7: 213–219Google Scholar
  48. Varanasi, U., Reichert, W. L., Stein, J. E., Brown, D. W., Sanborn, H. R. (1985). Bioavailability and biotransformation of aromatic hydrocarbons in benthic organisms exposed to sediment from an urban estuary. Envir. Sci. Technol. 19: 836–841Google Scholar
  49. Vighi, M., Calamari, D. (1985). QSARs for organotin compounds on Daphnia magna. Chemosphere (U.K.) 14: 1925–1932Google Scholar
  50. Widdows, J., Burns, K. A., Menon, N. R., Page, D. S., Soria, S. (1990). Measurement of physiological energetics (scope for growth) and chemical contaminants in mussels (Arca zebra) transplanted along a contamination gradient in Bermuda. J. exp. mar. Biol. Ecol. 138: 99–117Google Scholar
  51. Zaroogian, G. E., Heltshe, J. F., Johnson, M. (1985). Estimation of bioconcentration in marine species using structure-activity models. Envir. Toxic. Chem. 4: 3–12Google Scholar
  52. Zuolian, C., Jensen, A. (1989). Accumulation of organic and inorganic tin in blue mussel, Mytilus edulis, under natural conditions. Mar. Pollut. Bull. 20: 281–286Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • J. P. Meador
    • 1
  • U. Varanasi
    • 1
  • C. A. Krone
    • 1
  1. 1.Environmental Conservation Division, Northwest Fisheries Science Center, National Marine Fisheries ServiceNational Oceanic Atmospheric AdministrationSeattleUSA

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