Summary
The localization of the basement membrane components type IV collagen and laminin was investigated in seven-day-old mouse embryos (NMRI) fixed with formaldehyde, using an immunoperoxidase technique. Posttreatment of the embryos with TBS (trishydroxymethylaminomethane buffered saline) buffer was prerequisite for restoration of the antigenicity after fixation. The localization of the peroxidase (PO) positive reaction after treatment with anti-type IV collagen and anti-laminin antibodies in the embryos has been compared with results obtained after fixating embryos with the addition of tannic acid. Tannic acid stained the basement membrane of the ectodermal cell layer, in particular the lamina densa. After immunostaining for type IV collagen and laminin, a strong PO-positive reaction in the lamina densa of the ectodermal basement membrane was observed.
A basement membrane of the endodermal cell layer had not yet been formed at this developmental stage. In this region, which is where a basement membrane was to develop in later stages, a tannic acid positive material consisting of granules with a diameter of about 25 nm was found near the surface of the endoderm. Moreover, PO-positive patches were seen in this part of the embryo after staining for laminin as well as after staining for type IV collagen. These PO-positive patches were mainly localized in areas where mesodermal cells lay adjacent to the surface of the endodermal cell layer. No positive staining for type IV collagen and laminin was found in the cytoplasm of either cetodermal or endodermal cells.
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References
Banerjee SD, Cohn RH, Bernfield MR (1977) The basal lamina of embryonic salivary epithelia. Production by the epithelium and role in maintaining lobular morphology. J Cell Biol 73:445–463
Bernfield M, Banerjee SD (1982) The turnover of basal lamina glycosaminoglycan correlates with epithelial morphogenesis. Dev Biol 9012:291–305
Brownell AG, Bessem CC, Slavkin HC (1981) Possible functions of mesenchyme cell-derived fibronectin during formation of basal lamina. PNAS 78:3711–3715
Carlin B, Jaffe R, Bender B, Chung AE (1981) Entactin, a novel basal lamina-associated sulfated glycoprotein. J Biol Chem 256:5209
Csato W, Merker HJ (1983) Production and formation of the basement membrane in embryonic tissues of the mouse. Cell Tissue Res 228:85–98
Ekblom P, Miettinen M, Rapola J, Foidart JM (1982) Demonstration of laminin, a basement membrane glycoprotein, in routinely processed formalin-fixed human tissues. Histochemistry 7513:301–308
Gosslau B, Barrach HJ (1979) Enzyme-linked immunosorbent microassay for quantification of specific antibodies to collagen type I, II, III. J Immunol Meth 29:71–77
Grant MM, Cutts NR, Brody JS (1983) Alterations in lung basement membrane during fetal growth and type 2 cell development. Dev Biol 97:173–183
Hassel J, Robey PG, Barrach HJ, Wilszek J, Rennard SI, Martin GR (1980) Isolation of a heparan sulfate-containing proteoglycan from basement membrane. PNAS 77:4495–4498
Hay ED (1978) Role of basement membranes in development and differentiation. In: Kefalides NA (eds) Biology and chemistry of basement membrane. Academic Press, New York London, pp 119–136
Helman K (1980) Intracellular localisation of fibronectin using immunoperoxidase cytochemistry in light and electron microscopy. J Histochem Cytochem 28:1233–1241
Kerken R (1980) Eine lichtmikroskopische, histochemische, autoradiographische und elektronenmikroskopische Untersuchung der embryonalen Entwicklung der Maus vom Zeitpunkt der Mesodermentstehung bis zur Somitenformation. Habilitationsschrift, Freie Universität, Berlin
Herken R, Barrach HJ (1984) Immunohistochemische Lokalisation von Laminin und Kollagen Type IV im Glomerulum der Niere. Verh Anat Ges 78:167–169
Holund B, Clausen PP, Clemmensen I (1981) The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue. Histochemistry 72:291–299
Ikawa H, Trelstad RL, Hutson JM, Manganaro TF, Donahoe PK (1984) Changing patterns of fibronectin, laminin, type IV collagen, and a basement membrane proteolgycan during rat Mullerian duct regression. Dev Biol 102/1:260–263
Kühn K, Wiedemann H, Timpl R, Risteli J, Dieringer H, Voss T, Glanville RW (1981) Macromolecular structure of basement membrane collagens. Identification of 7S collagen as a crosslinking domain of type IV collagen. FEBS Lett 125:123–128
Laurie GW, Leblond CP, Martin GR (1982a) Intracellular localization of basement membrane precursors in the endodermal cells of the rat parietal yolk sac II. Immunostaining for type IV collagen and its precursors. J Histochem Cytochem 30/10:983–990
Laurie GW, Leblond CP, Martin GR, Silver MH (1982b) Intracelular localization of basement membrane precursors in the endodermal cells of the rat parietal yolk sac III. Immunostaining for laminin and its precursors. J Histochem Cytochem 30/10:991–998
Laurie GW, Leblond CP, Martin GR (1982c) Localization of type IV collagen, laminin, heparan sulfate proteoglycan, and fibronectin to the basal lamina of basement membranes. J Cell Biol 95/1:340–344
Leivo I, Vaheri A, Timpl LR, Wartiovaara J (1980) Appearance and distribution of collagens and laminin in the early mouse embryo. Dev Biol 76:100–114
Lunt DM, Seegmiller RE (1979) Differential localisation of (35S) sulfate within ectodermal basement membrane in relation to initiation of chick limb buds. JEEM 60:189–199
Mark H von der, Mark K von der, Gay S (1976) Study of differential collagen synthesis during development of the chick embryo by immunofluorescence I. Preparation of collagen type I and II specific antibodies and their application to early stages of the chick embryo. Dev Biol 48:237–249
Mayer Jr, BW, Hay ED, Hynes RO (1981) Immunocytochemical localization of fibronectin in embryonic chick trunk and area vasculosa. Dev Biol 82:267–286
Osman M, Ruch JV (1980) Secretion of basal lamina by trypsinisolated embryonic mouse molar epithelia cultured in vitro. Dev Biol 75:467–470
Sanders EJ (1979) Development of the basal lamina and extracellular materials in the early chick embryo. Cell Tissue Res 198:527–537
Sanders EJ (1982) Ultrastructural immunocytochemical localization of fibronectin in the early chick embryo. J Embryol Exp Morphol 71:155–170
Singer II (1979) The fibronexus: a transmembrane association of fibronectin-containing fibers and bundles of 5 nm microfilaments in hamster and human fibroblasts. Cell 16:675–685
Singley CT, Solursh M (1980) The use of tannic acid for the ultrastructural visualization of hyaluronic acid. Histochemistry 65:93–102
Thesleff I, Barrach HJ, Foidart JM, Vaheri A, Pratt RM, Martin GR (1981) Changes of distribution of type IV collagen, laminin, proteolgycan and fibronectin during mouse tooth development. Dev Biol 81:182–192
Timpl R, Rhode H, Robey PG, Rennard SI, Foidart JM, Martin GR (1979) Laminin-a glycoprotein from basement membranes. J Biol Chem 254:9933–9937
Timpl R, Wiedemann H, van Delden V, Furthmayr H, Kühn K (1981) A network model for the organization of type IV collagen molecules in basement membranes. Eur J Biochem 120:203–211
Wilson B, Nakane PK (1978) Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. In: Knapp W, Holubur K, Wick G (eds) Immunofluorescence and related staining techniques. Elsevier/North Holland: 219–224
Witschi E (1972) Characterization of developmental stages. In: Biology Data Book, second ed, Vol 1 Federation of American Societies for Experimental Biology:176–180
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Herken, R., Barrach, HJ. Ultrastructural localization of type IV collagen and laminin in the seven-day-old mouse embryo. Anat Embryol 171, 365–371 (1985). https://doi.org/10.1007/BF00347025
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DOI: https://doi.org/10.1007/BF00347025