Abstract
Two batches of experimental media containing 10% milk whey (sweet or sour) and 1% bovine serum supported the growth of BHK-21 cells in serial passages. The cell yield was greater than 2×106/ml at 48 h. The cells were susceptible to inoculation with foot and mouth disease (FMD) virus type ‘A’ and the virus titres obtained were comparable with those in Eagle's MEM-G with 10% serum. The use of milk whey could reduce the consumption of serum required for the growth of FMD virus in BHK-21 cells by up to 90%.
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References
Forman, A.J., 1974. A study of foot and mouth disease virus strains by complement fixation. A model for the fixation of complement by antigen/antibody mixtures. Journal of Hygiene (Cambridge), 72, 397–405
Griffith, J.B., 1972. Role of serum, insulin and amino acid concentration in contact inhibition of growth of human cells in culture. Experimental Cell Research, 75, 47–56
Hoskins, J.M., 1967. Virological Procedures, (Butterworth, London), 238–39
Keay, L., 1975. Autoclavable low-cost serum-free cell culture media. The growth of L cells and BHK cells on peptones. Biotechnology and Bioengineering, 17, 745–764
Moussa, A.A.M., Stouraitis, P., Ibrahim, M.H., Daoud, A. and Hussein, K., 1974. Foot and mouth disease vaccine production in BHK-21 C-13 cells in suspension in Egypt. Bulletin de l'Office International des Epizooties, 81, 1043–1054
Reed, L.V. and Muench, H., 1938. A simple method of estimating 50 per cent end point. American Journal of Hygiene, 27, 493
Saha, S.N., Nair, S.P. and Sen, A.K., 1982. Note on the growth of BHK-21 suspension cells in closed glass vessels. Indian Journal of Animal Sciences, 52, 356–357
Saha, S.N. and Sen, A.K., 1987. Studies on selection of suitable cell systems for adaptation, titration and plaque assay of foot and mouth disease virus type A. Indian Veterinary Journal, 64, 541–545
Sen, A.K. and Uppal, P.K., 1980. Use of whey in goat kidney cell culture of foot and mouth disease virus. Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases, 1, 6–10
Tomei, L.D. and Issel, C.J., 1975. Growth of immunogenicity of foot and mouth disease virus in baby hamster kidney cells adapted to and continuously grown in serum-free chemically defined media. Biotechnology and Bioengineering, 17, 765–778
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Saha, S.N., Sen, A.K. Milk whey as a serum supplement for the growth of FMD virus in BHK-21 cells. Veterinary Research Communications 13, 89–92 (1989). https://doi.org/10.1007/BF00346718
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DOI: https://doi.org/10.1007/BF00346718