Abstract
HLA class 11 molecules were isolated from mouse L cells transfected with a DR α gene and an allele, 52a, of locus DR β III from an HLA-homozygous cell line, AVL, of the DR3 haplotype. The isolated molecules were found to possess a new allospecificity, named TR81. This specificity behaved allelic to the previously described DR β III locus. The TR81 specificity was also present on the DR β I gene product of the DR3 haplotype. The nucleotide sequence of the gene encoding TR81 differs from TR81-negative DR β genes of the DRw52 family in only two codons, both located in the regions known to be involved in a gene conversion event. Consequently, the following conclusions can be formulated. (a) TR81 is a bi-locus specificity and allelic to TR22 only in its DR β III locus localization. (b) The TR81 specificity is the phenotypic counterpart of the gene conversion event which led to the generation of the DR β I gene of the DR3 haplotype. (c) One or both individual amino acid substitutions in the first domain of the DR β chain are responsible for the TR81 allospecificity. (d) Since TR81 is expressed on the DR β I chain of the DR3 haplotype, it is possible that TR81 and DR3 represent the same serological specificity.
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Angelini, G., de Preval, C., Gorski, J., and Mach, B.: High-resolution analysis of the human HLA-DR polymorphism by hybridization with sequence-specific oligonucleotide probes. Proc. Natl. Acad. Sci. U.S.A. 83: 4489–4493, 1986
Berdoz, J., Gorski, J., Termijtelen, A. M., Dayer, J. M., Irle, C., Schendel, D., and Mach, B.: Constitutive and induced expression of the individual HLA-DR beta and alpha chain loci in different cell types. J. Immunol., in press, 1987
Bontrop, R., Tilanus, M., Mikulski, M., van Eggermond, M., Termijtelen, A., and Giphafrt, M.: Polymorphisms within the HLA-DR3 haplotypes. I. HLA-DR polymorphisms detected at the protein and DNA levels are reflected by T-cell recognition. Immunogenetics 23: 401–405, 1986
Ferrara, G. B., Tosi, R., Longo, A., Castellani, A., Viviani, C., and Carminati, G.: A safe blood transfusion procedure for immunization against major histocompatibility complex determinants in man. Transplantation 26: 150–152, 1978
Gorski, J. and Mach, B.: Polymorphism of human la antigens: gene conversion between two DR beta loci results in a new HLA-D/DR specificity. Nature 322: 67–70, 1986
Gorski, J., Tosi, R., Strubin, M., Rabourdin-Combe, C., and Mach, B.: Serological and immunochemical analysis of the products of a single HLA DR-alpha and DR-beta chain gene expressed in a mouse cell line after DNA-mediated cotransformation reveals that the beta chain carries a known supertypic specificity. J. Exp. Med. 162: 105–116, 1985
Gorski, J., Tilanus, M., Giphart, M., and Mach, B.: Oligonucleotide genotyping shows that alleles at the HLA-DR β III locus of the DRw52 supertypic group segregate independently of known DR or Dw specificities. Immunogenetics 25: 79–83, 1987
Rabourdin-Combe, C. and Mach, B.: Expression of HLA-DR antigens at the surface of mouse L cells co-transfected with cloned human genes. Nature 303: 670–674, 1983
Rollini, P., Mach, B., and Gorski, J.: Linkage map of three HLA-DR beta chain genes: evidence for a recent duplication event. Proc. Natl. Acad. Sci. U.S.A. 82: 7197–7201, 1985
Rollini, P., Mach, B., and Gorski, J.: Characterization of an HLA-DR β pseudogene in the DRw52 supertypic group. Immunogenetics 25: 336–342, 1987
Tanigaki, N. and Tosi, R.: Assessment of the specificity of human Ia alloantisera and alloantigens by the use of radioiodinated human Ia antigens. Tissue Antigens 20: 1–21, 1982
Tanigaki, N., Tosi, R., Duquesnoy, R., and Ferrara, G. B.: Three Ia species with different structures and alloantigenic determinants in an HLA-homozygous cell line. J. Exp. Med. 157: 231–247, 1983a
Tanigaki, N., Tosi, R., Sagawa, K., Minowada, J., and Ferrara, G. B.: The distribution of DR5, MT2, and MB3 specificities on human Ia subsets. Immunogenetics 17: 371–386, 1983b
Tanigaki, N., Tosi, R., and Ferrara, G. B.: Serological discrimination of DR4 haplotypes by radioimmunoassay. Immunogenetics 23: 38–46, 1986
Tanigaki, N., Tosi, R., Strominger, J. L., and Cooper, J.: Immunochemistry of the HLA class II molecules isolated from a mouse cell transfected with DQ alpha and beta genes from a DR4 haplotype. Immunogenetics 26: 40–47, 1987
Termijtelen, A. M., Tilanus, M. G. J., Engelen, I., Koning, F., and van Rood, J. J.: Hum. Immunol., in press, 1987
Tosi, R., Tanigaki, N., de Preval, C., Gorski, J., and Mach, B.: Immunochemical analysis of a cell transfected with an HLA-DR gene reveals a new alloantigenic specificity within HLA-DRw52. Eur. J. Immunol. 16: 1603–1608, 1986
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Berte, C.C., Tanigaki, N., Tosi, R. et al. Serological recognition of HLA-DR allodeterminant corresponding to DNA sequence involved in gene conversion. Immunogenetics 27, 167–173 (1988). https://doi.org/10.1007/BF00346582
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DOI: https://doi.org/10.1007/BF00346582