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The early development of mouse embryos in vitro in medium supplemented with different batches of serum and bovine serum albumin

  • Biochemistry
  • Short Communication
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Abbreviations

BSA:

bovine serum albumin

DBS:

donor bovine serum

FBS:

fetal bovine serum

ECG:

equine chorionic gonadotrophin

hCG:

human chorionic gonadotrophin

IU:

international unit

References

  • Barnes, D and Sato, G., 1980. Serum-free cell culture: a unifying approach. Cell, 22, 649–655

    Google Scholar 

  • Biggers, J.D., Whitten, W.K. and Whittingham, D.G., 1971. The culture of mouse embryos in vitro. In: J.C.Daniel and J.Freeman (eds.), Methods for Mammalian Embryology, (W.H. Freeman and Co., San Francisco, CA, USA), 86–116

    Google Scholar 

  • Brinster, R.L., 1963. A method for in vitro cultivation of mouse ova from 2-cell to blastocyst. Experimental Cell Research, 32, 205–208

    Google Scholar 

  • Daniel, W., 1978. Chi-square test of independence and homogeneity. In: W.Daniel (ed.), Applied Non-Parametric Statistics, (H. Mifflin, Boston, MA, USA), 160–184

    Google Scholar 

  • Gray, C.W., Morgan, P.M. and Kane, M.T., 1992. Purification of an embryotrophic factor from commercial bovine serum albumin and its identification as citrate. Journal of Reproduction and Fertility, 94, 471–480

    Google Scholar 

  • McKierman, S.H. and Bavister, B.D., 1992. Different lots of bovine serum albumin inhibit or stimulate in vitro development of hamster embryos. In vitro Cellular and Developmental Biology, 28A, 154–156

    Google Scholar 

  • Mintz, B., 1967. Mammalian embryo culture. In: F.H.Witt and N.K.Wessels (eds), Methods in Developmental Biology, (Thomas Crowell, New York), 21–27

    Google Scholar 

  • Pratt, H.P., 1987. Isolation, culture and manipulation of preimplantation mouse embryos. In: M.Monk (ed.), Mammalian Development, A Practical Approach, (IRL Press, Oxford), 13–14

    Google Scholar 

  • Rorie, R.W., Miller, G.F., Nasti, K.B. and McNew, R.W., 1994. In vitro development of bovine embryos as affected by different lots of bovine serum albumin and citrate. Theriogenology, 42, 397–404

    Google Scholar 

  • SAS (1985). SAS Statistics Version 5. SAS User's Guide, (SAS Institute, Inc, Cary, NY, USA)

    Google Scholar 

  • Seidel, S.M. and Stringfellow, D.A., 1990. Certification and identification of the embryo. In: S.M.Seidel and D.A.Stringfellow (eds), Manual of the IETS, (International Embryo Transfer Society, Champaign, IL, USA), 12–59

    Google Scholar 

  • Tarkowski, A.K., 1966. An air-drying method for chromosome preparations from mouse eggs. Cytogenetics, 5, 394–400

    Google Scholar 

  • Tornesi, M.B., Palasz, A.T., DelCamp, M.R., Rousseaux, C.G., Archer, J.F. and Mapletoft, R.J., 1993. In vitro culture of preimplantation mouse embryos and day 12 limb-buds: Effects of serum and albumin. Reproductive Toxicology, 7, 623–630

    Google Scholar 

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Authors and Affiliations

  1. Department of Veterinary Pathology, WCVM, University of Saskatchewan, 52 Campus Drive, S7N 5B4, Saskatoon, Saskatchewan, Canada

    M. B. Tornesi & J. Archer

Authors
  1. M. B. Tornesi
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  2. J. Archer
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Tornesi, M.B., Archer, J. The early development of mouse embryos in vitro in medium supplemented with different batches of serum and bovine serum albumin. Veterinary Research Communications 20, 15–19 (1996). https://doi.org/10.1007/BF00346571

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  • DOI: https://doi.org/10.1007/BF00346571

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