Abstract
A monoclonal antibody specific for HLA-Bw4 was employed for the quantitative estimation of class I antigens on human peripheral blood lymphocytes and on the human macrophage cell line U 937. The epitopes reactive with the HLA-Bw4-specific antibody, which are present on different antigens coded by the HLA-A or the HLA-B locus, were characterized in terms of equilibrium and kinetic binding parameters. The level of expression of class I antigens on human lymphocytes was found to be in direct proportion to the gene dose. Variations between donors of the same phenotype were of minor importance. Estimation of the association constant, association rate constant, and half-life of dissociation for the interaction of the antibody with lymphocytes heterozygous for either HLA-A24, HLA-A32, HLA-A9, or HLA-Bw4 strongly suggested that the public antigenic determinant shared by these antigens is identical.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barnstable, C. J., Bodmer, W. F., Brown, G., Galfre, G., Milstein, C., Williams, A. F., and Ziegler, A.: Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens—new tools for genetic analysis. Cell 14: 9–20, 1978
Bishara, A., Nelken, D., Bonavida, B., and Brautbar, C.: Enzyme-linked immunosorbent assay for determination of HLA: Gene dose effect. Tissue Antigens 23: 284–289, 1984
Bradford, M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal. Biochem. 72: 248–254, 1976
Colombani, J. and Lepage, V.: HLA monoclonal antibody registry: Third listing. Tissue Antigens 24: 209–214, 1984
Cornish-Bowden, A. and Endrenyi, L.: Robust regression of enzyme kinetic data. Biochem. J. 234: 21–29, 1986
Cressie, N. A. C. and Keightley, D. D.: The underlying structure of the direct linear plot with application to the analysis of hormone-receptor interactions. J. Steroid Biochem. 11: 1173–1180, 1979
Cressie, N. A. C. and Keightley, D. D.: Analysing data from hormone-receptor assays. Biometrics 37: 235–249, 1981
Crothers, D. M. and Metzger, H.: The influence of polyvalency on the binding properties of antibodies. Immunochemistry 9: 341–357, 1972
Dower, S. K. and Segal, D. M.: Interaction of monoclonal antibodies with MHC class I antigens of mouse spleen cells. II. Levels of expression of H-2K, H-2D, and H-2L in different mouse strains. J. Immunol. 134: 431–435, 1985
Dower, S. K., Ozato, K., and Segal, D. M.: The interaction of monoclonal antibodies with MHC class I antigens on mouse spleen cells. I. Analysis of the mechanism of binding. J. Immunol. 132: 751–758, 1984
Frankel, M. E. and Gerhard, N.: The rapid determination of binding constants for antiviral antibodies by a radioimmunoassay. An analysis of the interaction between hybridoma proteins and influenza virus. Mol. Immunol. 16: 01–106, 1979
Halloran, P. F., Wadgymar, A., and Autenried, T.: The regulation of expression of major histocompatibility complex products. Transplantation 41: 413–420, 1986
Harris, H. W. and Gill, T. J.: Expression of class I transplantation antigens. Transplantation 42: 109–117, 1986
Konoeda, Y., Terasaki, P. I., Wakisaka, A., Park, M. S., and Mickey, M. R.: Public determinants of HLA indicated by pregnancy antibodies. Transplantation 41: 253–259, 1986
Kostyu, D. D., Cresswell, P., and Amos, D. B.: A public HLA antigen associated with HLA-A9, Aw32 and Bw4. Immunogenetics 10: 433–442, 1980
Laemmli, V. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685, 1970
Martin, D., Fauchet, R., Müller, G., Radka, S., Müller, C. P., Wernet, P., Ziegler, A., and Uchańska-Ziegler, B.: Expression of HLA-A and -B antigens on differentiating U-937 cells. Tissue Antigens 25: 235–246, 1985
Mason, D. W. and Williams, A. F.: The kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochem. J. 187: 1–20, 1980
McConahey, P. J. and Dixon, F. I.: A method for trace iodination of proteins for immunologic studies. Int. Arch. Allergy Appl. Immunol. 29: 185–189, 1966
Müller, C., Ziegler, A., Müller, G., Schnunter, F., and Wernet, P.: Monoclonal antibody (TU48) defining alloantigenic class I determinants specific for HLA-Bw4 and HLA-Aw23, -Aw24, as well as -Aw32. Hum. Immunol. 5: 269–281, 1982
N'Guyen, C., Sodoyer, R., Trucy, J., Strachan, T., and Jordan, B. R.: The HLA-Aw24 gene: Sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes. Immunogenetics 21: 479–489, 1985
Parham, P.: Monoclonal antibodies against two separate alloantigenic sites of HLA-B40. Immunogenetics 13: 509–527, 1981
Parham, P.: Antigenic determinants of the HLA-B7 molecule; Bw6- and B7-specific determinants are spatially separate. Immunogenetics 18: 1–16, 1983
Parham, P.: The binding of monoclonal antibodies to cell surface molecules. Quantitative analysis of the reactions and cross-reactions of an antibody (MB40.3) with four HLA-B molecules. J. Biol. Chem. 259: 13077–13083, 1984
Parham, P., Androlewics, M. J., Brodsky, F. M., Holmes, N. J., and Ways, J. P.: Monoclonal antibodies: Purification, fragmentation and application to structural and functional studies of class I MHC antigens. J. Immunol. Methods 53: 133–173, 1982
Radka, S. F., Kostyu, D. D., and Amos, D. B.: A monoclonal antibody directed against the HLA-Bw6 epitope. J. Immunol. 128: 2804–2806, 1982
Rose, M. L.: Immunoregulation of MHC antigen expression. Immunol. Today 6: 297–298, 1985
Rychlíková, M., Hinzová, E., Nosek, J., and Forejt, J.: Supertypic HLA-Bw4 antigen detected by a new monoclonal antibody. Exp. Clin. Immunogenet. 3: 88–97, 1986
Sanderson, A. R. and Welsh, I.: Properties of histocompatibility (HLA) determinants. I. Site density of antigens of the two HLA segregant series on peripheral human lymphocytes. Transplantation 17: 281–289, 1974
Seemann, G. H. A., Rein, R. S., Brown, C. S., and Ploegh, H. L.: Gene conversion-like mechanisms may generate polymorphism in human class I genes. EMBO J. 5: 547–552, 1986
Sundström, C. and Nilsson, K.: Establishment and characterization of human histiocytic lymphoma cell line U-937. Int. J. Cancer 17: 565–577, 1976
Trucco, M., De Petris, S., Garotta, G., and Ceppellini, R.: Quantitative analysis of cell surface HLA structures by means of monoclonal antibodies. Hum. Immunol. 3: 233–242, 1980
Ways, J. P. and Parham, P.: The binding of monoclonal antibodies to cell surface molecules. Quantitative analysis with immunoglobulin G against two alloantigenic determinants of the human transplantation antigen HLA-A2. Biochem. J. 216: 423–432, 1983
Ways, J. P., Coppin, H. L., and Parham, P.: The complete primary structure of HLA-Bw58. J. Biol. Chem. 260: 11924–11933, 1985
Ways, J. P., Rothbard, J. B., and Parham, P.: Amino acid residues 56 to 69 of HLA-A2 specify an antigenic determinant shared by HLA-A2 and HLA-B17. J. Immunol. 137: 217–222, 1986
Wong, D. M. and Mittal, K. K.: A murine monoclonal antibody specific for a novel broad antigen associated with HLA-Bw4. Tissue Antigens 27: 10–14, 1986
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nosek, J., Hinzová, E. & Vorlícěk, J. Quantitative estimation of HLA-A and HLA-B antigens carrying the Bw4 supertypic specificity in human peripheral blood lymphocytes. Immunogenetics 26, 273–281 (1987). https://doi.org/10.1007/BF00346522
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00346522