Abstract
Euchromatin DNA of one X chromosome in mammalian females, which becomes facultatively heterochromatinized, is known to replicate asynchronously late in S phase compared to its active homologue. In the females of a pygmy mouse species Mus dunni, which has prominent segment of constitutive heterochromatin as the short arm of its submetacentric X chromosome, we have observed asynchronous replication of c-heterochromatin arm as well, predominant number of cells showing the segment associated with the facultatively heterochromatic X to be terminating later. The preferential later termination of replication of the c-heterochromatic arm on the “lyonized X” appears to be due to the influence of facultative heterochromatin on the adjacent constitutive heterochromatin.
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References
Cattanach, B.M., Isaacson, J.H.: Genetic control over the inactivation of autosomal genes attached to the X chromosome. Z. Vererb. lehre 96, 313–323 (1965)
Comings, D.E.: Quantitative autoradiography of heterochromatin replication in Microtus agrestis. Chromosoma (Berl.) 29, 434–445 (1970)
Galton, M., Holt, S.F.: Asynchronous replication of the mouse sex chromosomes. Exp. Cell Res. 37, 111–116 (1965)
German, J.: The pattern of DNA synthesis in the chromosomes of human blood cells. J. Cell Biol. 1, 37–55 (1964)
Goto, K., Akematsu, T., Shimazu, H., Sujiyama, T.: Simple differential Giemsa staining of sister chromatids after treatment with photosensitive dyes and exposure to light and mechanism of staining. Chromosoma (Berl.) 53, 223–230 (1975)
Kanda, N.: A new differential technique for staining the heteropycnotic X chromosome in female mice. Exp. Cell Res. 80, 463–467 (1973)
Lima de Faria, A., Bianchi, N.O., Nowell, P.: Replication in chromosomes from chorionic granulocytic leukemia. J. Cell Biol. 23, 107 (1964)
Miller, O.J., Schnedl, W., Alien, J., Erlanger, B.F.: 5 methyl cytosine localised in mammalian constitutive heterochromatin. Nature (Lond.) 251, 636–637 (1974)
Mohandas, T., Sparkes, R.S., Shapiro, L.J.: Reactivation of an inactive human X chromosome: evidence for X inactivation by DNA methylation. Science 211, 393–396 (1981)
Nesbitt, M.N., Gartler, S.M.: Replication of the mouse sex chromosomes early in the S period. Cytogenetics 9, 212–221 (1970)
Riggs, A.D.: X inactivation, differentiation and DNA methylation. Cytogenet. Cell Genet. 14, 9–25 (1975)
Sandberg, A.A., Takagi, N., Schmidt, M.L., Bross, I.D.T.: Chronology and pattern of human chromosome replication. IX Metasynchronous replication in homologs. Cytogenetics 7, 298–332 (1968)
Schempp, W.: Asynchrony in late replication between homologous autosomes in primary cultures of Chinese hamster fibroblasts. Chromosoma (Berl.) 79, 199–206 (1980)
Schmid, W., Leppert, M.F.: Rates of DNA synthesis in heterochromatic and euchromatic segments of the chromosome complements of two rodents. Cytogenetics 8, 125–135 (1969)
Schneider, L.K., Rieke, W.O.: DNA replication patterns and chromosomal protein synthesis in opossum lymphocytes in vivo. J. Cell Biol. 33, 497–509 (1967)
Solage, A., Cedar, H.: Organization of 5 methylcytosine in chromosomal DNA. Biochemistry 17, 2934–2938 (1978)
Taylor, J.H.: Asynchronous duplication of chromosomes in cultured cells of Chinese hamster. J. biophys. biochem. Cytol. 7, 455–464 (1960)
Zabel, B.U., Baumann, W.A., Pirntke, W., Gerhard-Ratschow, K.: X inactivation pattern in three cases of X/Autosome translocation. Amer. J. med. Genet. 1, 309–317 (1978)
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Sen, S., Sharma, T. Asynchronous replication of constitutive heterochromatin on X chromosomes in female Mus dunni . Chromosoma 85, 119–126 (1982). https://doi.org/10.1007/BF00344598
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DOI: https://doi.org/10.1007/BF00344598