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The male-derived genome after sperm-egg fusion: Spatial distribution of chromosomal DNA and paternal-maternal genomic association

Chromosoma volume 100, pages 262–266 (1991)Cite this article

Abstract

After fusion with an egg, the haploid genome of a mammalian sperm expands from a volume of a few cubic microns to a volume of several thousand cubic microns in a fully developed zygote pronucleus. Using fluorescent in situ hybridization we studied two aspects of the chromatin organization of the sperm-derived genome during this process in a model system involving fusion of human sperm with hamster eggs. We found that (a) from the beginning of sperm head decondensation to early pronuclear stages, the hybridization signal of a probe targeted to the satellite III heterochromatin of chromosome 1 appeared as an irregularly shaped domain. In fully developed pronuclei, the signal became a fiber as much as 20-fold more extended compared with interphase somatic nuclei. The signal appeared as a string of non-uniformly distributed “beads” with interspersed gaps. A similar spatial distribution and appearance recurred after the first cleavage division. (b) Male-and female-derived genomes assembled non-randomly on the first mitotic spindle, and continued to be sequestered from each other in the nuclei of two-cell stages.

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Affiliations

  1. Lawrence Livermore National Laboratory, Biomedical Sciences Division, University of California, P.O. Box 808, L-452, Livermore, CA, 94550, USA

    B. F. Brandriff, L. A. Gordon, R. Segraves & D. Pinkel

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  1. B. F. Brandriff
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  2. L. A. Gordon
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  3. R. Segraves
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  4. D. Pinkel
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by J.B. Rattner

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Brandriff, B.F., Gordon, L.A., Segraves, R. et al. The male-derived genome after sperm-egg fusion: Spatial distribution of chromosomal DNA and paternal-maternal genomic association. Chromosoma 100, 262–266 (1991). https://doi.org/10.1007/BF00344160

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  • DOI: https://doi.org/10.1007/BF00344160

Keywords

  • Hybridization Signal
  • Mitotic Spindle
  • Human Sperm
  • Haploid Genome
  • Sperm Head