Abstract
Two alloantisera against hybridoma-derived IgE detected allotypic determinants expressed on the murine s chain. An antiserum raised in BALB/c mice against monoclonal IgE of C57BL/6 origin reacted exclusively with IgE of strains having Igh-1b (IgG2a) allotype. The second antiserum, C57BL/6 anti-BALB/c monoclonal IgE, reacted with IgE of strains having Igh-1a, Igh-1d, Igh-1e and Igh-1j allotypes. The genetic studies of (BALB/c x C57BL/6)F1 and backcross F2 animals indicated that the locus controlling the IgE allotype is linked to the Igh-1 locus. This was further confirmed by the possession of respective IgE allotypes by Igh-C congenic mice, BALB/c and BAB-14, C3H.SW/Hz and CWB/Hz. Thus, the allotype detected on the ε chain is controlled by the seventh murine immunoglobulin allotype locus, and should be designated as the Igh-7 allotype.
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Abbreviations
- PCA:
-
passive cutaneous anaphylaxis
- RID:
-
radioimmunodiffusion
- i.p.:
-
intraperitoneally
- EA:
-
egg albumin
- Igh-C:
-
immunoglobulin heavy chain constant region locus
- DNP:
-
2,4-dinitrophenyl
- PBS:
-
phosphate-buffered saline
- NMS:
-
normal mouse serum
- KLH:
-
keyhole limpet hemocyanin
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Visiting investigator supported by the Scientific and Humanistic Development Council from the Central University of Venezuela, currently at the following address: Consejo de Desarrollo Cientifico y Universidad Central de Venezuela, Av. Principal Urb. La Floresta Ota., Silenia Caracas, Venezuela.
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Borges, M.S., Kumagai, Y., Okumura, K. et al. Allelic polymorphism of murine IgE controlled by the seventh immunoglobulin heavy chain allotype locus. Immunogenetics 13, 499–507 (1981). https://doi.org/10.1007/BF00343718
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DOI: https://doi.org/10.1007/BF00343718