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Molecular analysis distinguishes two HLA-DR3-bearing major histocompatibility complex extended haplotypes

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Abstract

We detected restriction fragment length polymorphisms that distinguish the extended haplotype HLA-B8,DR3,SCO1 from HLA-B18,DR3,F1C30 at the DR beta and DQ beta loci with five of seven restriction endonucleases used. One set of restriction fragments was always found on HLA-B8,DR3,SCO1 and associated with DRw52a, while the other was present on HLA-B18, DR3,F1C30 and correlated with DRw52b (the gene encoding the subtype of DRw52 associated with the BO1 or LB-Q1 antigen). Furthermore, using a full-length DQ beta gene probe, we found division in the DQw2 haplotype, in which DQw2a always associated with HLA-B8, DR3,SCO1, while DQw2b always occurred with HLA-B18,DR3,F1C3O. Our evidence thus indicates that serologically defined HLA-DR3, HLA-DRw52, and HLA-DQw2 are each produced by two structurally very different sets of genes, one set occurring in HLA-B8, DR3,SCO1, and the other in HLA-B18,DR3,F1C30.

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Abbreviations

BSA:

bovine serum albumin

MHC:

major histocompatibility complex

EDTA:

ethylenediaminetetraacetic acid

SDS:

sodium dodecyl sulfate

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Authors and Affiliations

  1. The Center for Blood Research, Boston, Massachusetts, USA

    Mark Z. Wescott, Z. L. Awdeh, Edmond J. Yunis &  Chester A. Alper

  2. Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA

    Z. L. Awdeh & Edmond J. Yunis

  3. Dana-Farber Cancer Institute, Boston, Massachusetts, USA

    Edmond J. Yunis

  4. Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA

    Chester A. Alper

  5. Department of Medicine, The Children's Hospital, Boston, Massachusetts, USA

    Chester A. Alper

Authors
  1. Mark Z. Wescott
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  2. Z. L. Awdeh
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  3. Edmond J. Yunis
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  4. Chester A. Alper
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Wescott, M.Z., Awdeh, Z.L., Yunis, E.J. et al. Molecular analysis distinguishes two HLA-DR3-bearing major histocompatibility complex extended haplotypes. Immunogenetics 26, 370–374 (1987). https://doi.org/10.1007/BF00343707

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  • DOI: https://doi.org/10.1007/BF00343707

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