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Antigenic determinants shared between HLA-A, −B, −C antigens and H-2 class I molecules modified by bovine beta-2 microglobulin

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Abstract

The specificity of the mouse class I-specific antibody COB6-3 was examined in detail. It was found to react with the mouse class I molecules H-2Db, Kd, and Qa-2, and with human HLA-A, −B, −C antigens. The specificity pattern of COB6-3, despite its different origin, was similar to that of the monomorphic HLA class I-specific antibody W6/32. Cross-inhibition studies show that on human cells the antigenic determinants recognized by the two antibodies are situated close together and may be identical. On mouse cells, reactivity of both antibodies was generated upon replacement of mouse beta-2 microglobulin (B2m) with its bovine counterpart, but differences in specificity were observed using human B2m.

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Abbreviations

B2m:

beta-2 microglobulin

BSA:

bovine serum albumin

FCS:

fetal calf serum

FITC:

fluorescein isothiocyanate

MHC:

major histocompatibility complex

PBL:

peripheral blood lymphocytes

PBS:

phosphate-buffered saline

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. Rheumaforschungsinstitut, Rheumaklinik Aachen, Burtscheider Markt 24, D-5100, Aachen, Germany

    Rudolf Mierau & Ekkehard Genth

  2. Institute for Immunology and Genetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-6900, Heidelberg, Germany

    Peter J. Robinson

  3. National Institute for Medical Research, The Ridgeway, NW7 1AA, Mill Hill, London, UK

    Arnold R. Sanderson

  4. Institute for Genetics, University of Cologne, Weyertal 121, D-5000, Köln 41, Germany

    Matthias Cramer

Authors
  1. Rudolf Mierau
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  2. Peter J. Robinson
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  3. Arnold R. Sanderson
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  4. Ekkehard Genth
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  5. Matthias Cramer
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Mierau, R., Robinson, P.J., Sanderson, A.R. et al. Antigenic determinants shared between HLA-A, −B, −C antigens and H-2 class I molecules modified by bovine beta-2 microglobulin. Immunogenetics 26, 351–355 (1987). https://doi.org/10.1007/BF00343703

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  • DOI: https://doi.org/10.1007/BF00343703

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