Summary
A tenfold increase in the spontaneous reversion frequency of two of six lacZ frameshift mutations tested was observed in strains containing the following DNA polymerase I mutations-polA1, polA5, polA6, polAex1, res-3 and resA1. Reconstruction experiments indicated that this increase was not the result of a selective effect. Only a fourfold increase in frameshift mutations was found in strains containing a polA107 mutation. Both the polAex1 and polA107 mutations result in defective 5′ to 3′ exonuclease activity and do not affect polymerizing activity, but have different effects on frameshift mutations. A polA mutation on the chromosome induced frameshift mutations in a gene on an F episome. None of three auxotrophic mutations studied showed high frequency reversion in the presence of the polA1 or polA6 mutations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berger, H., Brammer, W. J., Yanofsky, C.: Spontaneous and ICR191-A induced frameshift mutations in the A gene of Escherichia coli tryptophan synthetase. J. Bact. 96, 1672–1679 (1968)
Calender, R., Lindquist, B., Sironi, G., Clark, A. J.: Characterization of REP− mutants and their interaction with P2 phage. Virology 40, 72–83 (1970)
DeLucia, P., Cairns, J.: Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature (Lond.) 224, 1164–1166 (1969)
Drake, J. W.: The genetic control of spontaneous and induced mutation rates in bacteriophage T4. Genetics (Suppl.) 73, 45–64 (1974)
Glickman, B. W.: The role of DNA polymerase I in pyrimidine dimer excision and repair replication in Escherichia coli K12 following ultraviolet irradiation. Biochim. biophys. Acta (Amst.) 335, 115–122 (1974)
Glickman, B. W., van Sluis, C. A., Heijneker, H. L., Rörsch, A.: A mutant of Escherichia coli K-12 deficient in the 5′→3′ exonucleolytic activity of DNA polymerase I. I. General characterization. Molec. gen. Genet. 124, 69–82 (1973)
Goebel, W., Schrempf, H.: Replication of the minicircular DNA of E. coli 15 is dependent on DNA polymerase I but independent of DNA polymerase III. Biochem. biophys. Res. Commun. 49, 591–600 (1972)
Gottesman, M. M., Hicks, M. L., Gellert, M.: Genetics and function of DNA ligase in Escherichia coli. J. molec. Biol. 77, 531–547 (1973)
Hall, R. M., Brammer, W. J.: Increased spontaneous mutation rates in mutants of E. coli with altered DNA polymerase III. Molec. gen. Genet. 121, 271–276 (1973)
Heijneker, H. L., Ellens, D. J., Tjeerde, R. H., Glickman, B. W., van Dorp, B., Pouwels, P. H.: A mutant of Escherichia coli K-12 deficient in the 5′→3′ exonucleolytic activity of DNA polymerase I. II. Purification and properties of the mutant enzyme. Molec. gen. Genet. 124, 83–96 (1973)
Hoess, R. H., Herman, R. K.: Segregation of induced frameshift mutations and the sequence of gene replication in Escherichia coli K-12. Genetics 74, 227–242 (1973)
Ishii, Y., Kondo, S.: Spontaneous and radiation-induced deletion mutations in Escherichia coli strains with different DNA repair capacities. Mutation Res. 16, 13–25 (1972)
Kato, T.: Excision repair characteristics of recB − res−and uvrC − strains of Escherichia coli. J. Bact. 112, 1237–1246 (1972)
Kato, T., Kondo, S.: Genetic and molecular characteristics of x-ray sensitive mutants of Escherichia coli defective in repair synthesis. J. Bact. 104, 871–881 (1970)
Kelley, W. S., Whitfield, H. J.: Purification of an altered DNA polymerase from an E. coli strain with a pol mutation. Nature (Lond.) 230, 33–36 (1971)
Kingsbury, D. T., Helinski, D. R.: DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem. biophys. Res. Commun. 41, 1538–1544 (1970)
Kondo, S.: Evidence that mutations are induced by errors in repair and replication. Genetics (Suppl.) 73, 109–122 (1973)
Konrad, E. B., Lehman, I. R.: A conditional lethal mutant of Escherichia coli K-12 defective in the 5′→3′ exonuclease associated with DNA polymerase I. Proc. nat. Acad. Sci. (Wash.) 71, 2048–2051 (1974)
Kornberg, A.: DNA polymerase I. In: DNA synthesis, p. 67–122. San Francisco: W. H. Freeman and Company, 1974
Kuempel, P. L., Veomett, G. E.: A possible function of DNA polymerase in chromosome replication. Biochem. biophys. Res. Commun. 41, 973–980 (1971)
Lehman, I. R., Chien, J. R.: Persistence of deoxyribonucleic acid polymerase I and its 5′→3′ exonuclease activity in polA mutants of Escherichia coli K-12. J. biol. Chem. 248, 7717–7723 (1973)
Low, B.: Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. J. Bact. 113, 792–812 (1973)
Mattern, I. E., Houtman, P. C.: Properties of uvrE mutants of Escherichia coli K-12. II. Construction and properties of pol and rec derivatives. Mutation Res. 25, 281–287 (1974)
Muzycka, N., Poland, R. L., Bessman, M. J.: Studies on the biochemical basis of spontaneous mutation. I. A comparison of the deoxyribonucleic acid polymerases of mutator, antimutator and wildtype strains of bacteriophage T4. J. biol. Chem. 247, 7116–7122 (1972)
Newton, A., Isolation and characterization of frameshift mutations in the lac operon. J. molec. Biol. 49, 589–601 (1970)
Newton, A., Masys, D., Leonardi, E., Wygal, D.: Association of induced frameshift mutagenesis and DNA replication in Escherichia coli. Nature (Lond.) New Biol. 236, 19–22 (1972)
Okazaki, R., Arisawa, M., Sugino, A.: Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants. Proc. nat. Acad. Sci. (Wash.) 68, 2954–2957 (1971)
Riddle, D. L., Roth, J. R.: Frameshift suppressors. III. Effects of suppressor mutations on transfer RNA. J. molec. Biol. 66, 495–506 (1972)
Roth, J. R.: Frameshift mutations. Ann. Rev. Genet. 8, 319–345 (1974)
Siegel, E. C.: Ultraviolet-sensitive mutator strain of Escherichia coli K-12. J. Bact. 113, 145–160 (1973)
Siegel, E. C., Kamel, F.: Reversion of frameshift mutations by mutator genes in Escherichia coli. J. Bact. 117, 994–1001 (1974)
Sirnov, G. B., Filkova, E. V., Skavronskaya, A. G., Saenko, A. S., Sinzinis, B. I.: Loss and restoration of viability of E. coli due to combinations of mutations affecting DNA polymerase I and repair activities. Molec. gen. Genet. 121, 139–150 (1973)
Speyer, J. F.: Mutagenic DNA polymerase. Biochem. biophys. Res. Commun. 21, 6–8 (1965)
Streisinger, G., Okada, Y., Emrich, J., Newton, J., Tsugita, A., Terzaghi, E., Inouye, M.: Frameshift mutations and the genetic code. Cold Spr. Harb. Symp. quant. Biol. 31, 77–84 (1966)
Taylor, A. L., Trotter, C. D.: Linkage map of Escherichia coli strain K-12. Bact. Rev. 36, 504–524 (1972)
Author information
Authors and Affiliations
Additional information
Communicated by B. A. Bridges
Rights and permissions
About this article
Cite this article
Vaccaro, K.K., Siegel, E.C. Increased spontaneous reversion of certain frameshift mutations in DNA polymerase I deficient strains of Escherichia coli . Molec. gen. Genet. 141, 251–262 (1975). https://doi.org/10.1007/BF00341803
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00341803