Summary
Mutants of the dnaA, dnaC, dnaD, polC, dnaF and dnaG gene loci were tested for their capacity for colicinogenic plasmid E1 (ColE1) replication at a non-permissive temperature. It was found that ColE1 replication was independent of the dnaA gene function and dependent on dnaC, D, F and G. ColE1 replication in the polC mutant E486 continued for several hours but at a greatly reduced rate. No effect was found of the dnaG mutation on thymine-deprivation-induced “priming” of ColE1 replication at the non-permissive temperature. The mutants also were tested for aberrant replication intermediates of plasmid DNA as well as a temperature sensitive supercoiled DNA-protein relaxation complex. RNA-containing supercoils were found to accumulated in a polC mutant also blocked for protein synthesis.
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Collins, J., Williams, P. & Helinski, D.R. Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication. Molec. gen. Genet. 136, 273–289 (1975). https://doi.org/10.1007/BF00341713
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DOI: https://doi.org/10.1007/BF00341713