Summary
The RNA polymerase inhibitor, lomofungin has been used to determine the half life of specific synthetic capacities (invertase and α-glucosidase) as well as that for gross protein synthesis. In both cases the studies conclude that cognate messenger RNAs decay with a half life of approximately 20 minutes. This antibiotic has been used to determine the half life of allophanate hydrolase specific synthetic capacity. We find that it decays with a half life of about three minutes; a value that agrees with the decay rates of allophanate hydrolase synthetic capacity following removal of inducer. These observations argue that mRNA may be metabolized by two separate routes in Saccharomyces.
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Communicated by W. Maas
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Lawther, R.P., Phillips, S.L. & Cooper, T.G. Lomofungin inhibition of allophanate hydrolase synthesis in Saccharomyces cerevisiae . Molec. gen. Genet. 137, 89–99 (1975). https://doi.org/10.1007/BF00341675
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DOI: https://doi.org/10.1007/BF00341675