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Expansive growth of the nuclear envelope and formation of mitochondria in ganglionic neuroblasts

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Zusammenfassung

Während des 4. und 5. Bruttages differenzieren sich im lateroventralen Abschnitt der Spinalganglien des Hühnerembryos viele Neuroblasten. In diesen Neuroblasten nimmt das Chondriom stark zu und man findet gleichzeitig folgende Strukturen: a) Rollen, aus Membranen gebildet, die einen komplizierten, von Fall zu Fall verschiedenen Verlauf aufweisen. Die Rollen setzen sich in die äußere Nuclearmembran fort. Zwischen ihren Membranen liegen elektronenoptisch dichte, den Ribosomen ähnelnde Granula. b) Membranrollen, deren peripherer halbmondförmiger Abschnitt feinkörniges Material enthält. c) Rollen, deren Membranen sich in die Außenmembran und die Cristae umfangreicher, unregelmäßig geformten Mitochondrien fortsetzen. d) Übergangsformen zwischen denen unter b) und c) beschrieben.

Die äußere Nuclearmembran, die Membranen der beschriebenen Rollen und der Mitochondrien weisen die gleiche Dicke auf. Die Membranen, welche eine der unter a) geschilderten Rollen bilden und jene, die sich in einem der umfangreichen Mitochondrien befinden (c), haben Oberflächen von gleicher Größenordnung.

Unter den verschiedenen Hypothesen zur Interpretation der Befunde verdient folgende den Vorzug, da sie eine klare Deutung aller gewonnenen Befunde erlaubt: die äußere Nuclearmembran dehnt sich aus und führt auf diese Weise zur Entstehung einer Membranrolle. In dieser Rolle bildet sich sodann ein körniges Material, den Vorläufer der MitochondrienMatrix. Die Menge des körnigen Materials nimmt zu, gleichzeitig wickeln sich nach und nach die Membranenrollen ab und bilden die Hülle und die Cristae eines umfangreichen Mitochondrions. Letzteres teilt sich endlich in mehrere kleinere Mitochondrien auf. Während der Neuroblastendifferenzierung der Spinalganglien des Hühnerembryos geht also die Bildung von Mitochondrien von der Nuclearhülle aus.

Summary

Many neuroblasts undergo differentiation in the lateroventral region of spinal ganglia in chick embryos at the 4th to 5th incubation day. In these neuroblasts a striking increase in the chondriome has been recorded, and the following structures have been observed. a) Whorls of membranes which are continuous with the outer nuclear membrane: ribosomelike granules lie in the intramembranous spaces of these whorls, b) Membranous whorls with a peripheral portion, crescent-shaped in the sections, containing a minute granular material, c) Membranous whorls which are continuous with the membranes of the envelope, and of the cristae of large, irregular mitochondria. d) Formations which are transitional in structure between the ones mentioned above.

No significant differences were recorded of the thickness of the outer nuclear membrane, of the whorls' membranes, and of the mitochondrial membranes. Beside, the mean values of the total area of the membranes of each whorl of the type listed under a), and of the total area of the membranes of large mitochondria, such as listed under c), are of the same order of magnitude.

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Authors and Affiliations

  1. Institute of Human Anatomy (Laboratory of Neurocytology “P. Rando”), University of Milano, Italy

    Ennio Pannese

  2. Department of Histology and Embryology, University of Bari, Italy

    Ennio Pannese

  3. Istituto di Anatomia Umana dell'Università, Via Mangiagalli, 31, Milano, Italy

    Ennio Pannese

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Research supported by a C.N. R. grant.

I wish to thank Prof. R. Amprino and Prof. J. D. Robertson for much useful discussion and criticism, Dr. phis. S. de Petris for the help given in the statistical evaluation of the data, Mrs. G. Fiori for technical assistance, and Miss A. Bertolasi for drawing Fig. 17.

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Pannese, E. Expansive growth of the nuclear envelope and formation of mitochondria in ganglionic neuroblasts. Zeitschrift für Zellforschung 72, 295–324 (1966). https://doi.org/10.1007/BF00341538

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