Summary
The protein granules secreted by the mammary gland and first revealed by electron microscopy can be seen in the light microscope, if appropriate techniques are emploied.
Resumé
Les grains de protéine sécrétés par la glande mammaire, révélés par le microscope électronique, peuvent s'individualiser au microscope optique à condition d'employer une technique assez fine.
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Bargmann, W., u. A. Knoop: Über die Morphologie der Milchsekretion. Licht- und elektronenmikroskopische Studien an der Milchdrüse der Ratte. Z. Zellforsch. 49, 344–388 (1959).
Bennett, H. S., and J. H. Luft: S-collidine as a basis for buffering fixatives. J. biophys. biochem Cytol. 6, 113–114 (1959).
Dalton, A. J., and M. D. Felix: A study of the Golgi substance and ergastoplasm in a series of mammalian cell types. In: Fine structure of cells. Symposium, 8th Int. Congr. of cell Biology, Leiden 1954, Groningen (Netherl.): Nordhoff 1955, p. 274–293.
Glauert, A. H., and R. H. Glauert: Araldite as embedding medium for electron microscopy. J. biophys. biochem. Cytol. 4, 191–194 (1958).
Grynfeltt, J.: Etude histologique des phénomènes sécrétoires de la glande mammaire. Thèse médecine Montpellier 1936.
Hollmann, K. H.: L'ultrastructure de la glande mammaire normale de la souris en lactation. J. Ultrastruct. Res. 2, 423–443 (1959).
Ito, S., and R. J. Winchester: The fine structure of the gastric mucosa in the bat. J. Cell Biol. 16, 541–577 (1963).
Marinozzi, V.: Silver impregnation of ultrathin sections for electron microscopy. J. biophys. biochem. Cytol. 9, 121–134 (1961).
Millonig, G.: Advantages of a phosphate buffer for OsO4 solutions in fixation. J. appl. Phys. 32, 16–37 (1961).
Palade, G.E.: A study of fixation for electron microscopy. J. exp. Med. 95, 285–298 (1952).
Sabatini, D. D., H. Bensch, and J. R. Barrnett: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell. Biol. 17, 19–58 (1963).
Verley, J. M., et K. H. Hollmann: Synthèse et réabsorption des protéines dans la glande mammaire en stase. Etude autoradiographique au microscope électronique. Z. Zellforsch. 75, 605–610 (1966).
Wellings, S. R., B. W. Grunbaum, and K. B. Deome: Electron microscopy of milk secretion in the mammary gland of the C3H/Crgl mouse. II. Identification of fat and protein particles in milk and in tissue. J. nat. Cancer Inst. 25, 423–437 (1960).
—, and J. R. Philp: The function of the Golgi apparatus in lactating cells of the BALB/cCrgl mouse. An electron microscopic and autoradiographic study. Z. Zellforsch. 61, 871–882 (1964).
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Avec l'aide du Centre National de la Recherche Scientifique et de l'Institut National de la Santé et de la Recherche Médicale.
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Hollmann, K.H., Verley, J.M. Individualisation au microscope optique des grains de proteine secretes par la glande mammaire. Zeitschrift für Zellforschung 75, 601–604 (1966). https://doi.org/10.1007/BF00341517
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DOI: https://doi.org/10.1007/BF00341517