Summary
To elucidate the in vivo function of the J gene of microvirid (isometric) phages, we isolated several strains carrying a double mutation in J and H genes from phage α3 and then constructed single mutants each having an amber codon in the J gene. The J mutants could not multiply in suppressor-less hosts and were deficient in single-stranded progeny DNA synthesis. Nucleotide sequences of the wild-type and mutant α3 J genes were analyzed to determine the mutation sites. The amino acid sequence of the J gene was also deduced from the nucleotide sequence and compared with those of ϕX174 and G4.
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Communicated by M. Takanami
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Kodaira, Ki., Taketo, A. Isolation and some properties of bacteriophage α3 gene J mutant. Mol Gen Genet 195, 541–543 (1984). https://doi.org/10.1007/BF00341460
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DOI: https://doi.org/10.1007/BF00341460