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The permeability of blood vessels in the guinea pig cochlea

II. Vessels in the spiral ligament and the stria vascularis

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Summary

The permeability of blood vessels in the stria vascularis, spiral prominence and spiral ligament has been investigated using the horseradish peroxidase (HRP) tracer technique. The distribution of the marker enzyme was studied 1/2-60 min after intravenous injection. The tissue was fixed in situ by vascular perfusion.

In the Stria vascularis already 1/2 min after the injection vesicular transport across endothelial cells is seen to have occurred and the tracer can be observed in the intercellular spaces. Perfusion fixation permits the demonstration of concentration gradients.

The diffusion of HRP is limited by barrier systems, represented by basal cells and their junctional complexes towards the perilymphatic space, and by marginal cells and their junctional complexes towards the endolymphatic space. Identical distribution patterns are observed up to 60 min after HRP injection. Intermediate cells phagocytize HRP.

In the spiral ligament and spiral prominence, uptake of HRP into endothelial cells is found in small amounts 5 min after injection of the tracer; in the surrounding fibrocytes some vesicles filled with reaction product are observed later than 20 min following the injection.

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Authors and Affiliations

  1. Institute of Anatomy, University of Cologne, Cologne, Germany

    Karin Gorgas & Klaus Jahnke

  2. Department of Otorhinolaryngology, University of Cologne, Cologne, Germany

    Karin Gorgas & Klaus Jahnke

  3. Hals-, Nasen- und Ohrenklinik, Universität zu Köln, D-5000, Köln 41, Federal Republic of Germany

    Klaus Jahnke

  4. Anatomisches Institut der Universität zu Köln, D-5000, Köln 41, Federal Republic of Germany

    Karin Gorgas

Authors
  1. Karin Gorgas
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  2. Klaus Jahnke
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Additional information

Directors: Prof. Dr. R. Ortmann, Prof. Dr. B. Kummer

Director: Prof. Dr. Dr. F. Wustrow

This work was supported by a grant of the “Deutsche Forschungsgemeinschaft”, project “receptor physiology.”

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Gorgas, K., Jahnke, K. The permeability of blood vessels in the guinea pig cochlea. Anat. Embryol. 146, 33–42 (1974). https://doi.org/10.1007/BF00341381

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  • DOI: https://doi.org/10.1007/BF00341381

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