Summary
DNA methyltransferase activity is not normally found in yeast. To investigate the response of Saccharomyces cerevisiae to the presence of methylated bases, we introduced the Bacillus subtilis SPR phage DNA-[cytosine-5] methyltransferase gene on the shuttle vector, YEp51. The methyltransferase gene was functionally expressed in yeast under the control of the inducible yeast GAL10 promoter. Following induction we observed a time-dependent methylation of yeast DNA in RAD + and rad2 mutant strains; the rad2 mutant is defective in excision-repair of UV-induced DNA damage. Analysis of restriction endonuclease digestion patterns revealed that the relative amount of methylated DNA was greater in the excision defective rad2 mutant than in the RAD + strain. These data indicate that the yeast excision-repair system is capable of recognizing and removing m5C residues.
References
Adams RLP, Burdon RH (1985) Methylation and its relationship with transcription. In: Rich A (ed) Molecular biology of DNA methylation. Springer, New York, pp 115–161
Antequera FM, Tamame J, Villaneuva JR, Santos T (1984) J Biol Chem 259:8033–8036
Antequera FM, Tamame J, Villaneuva JR, Santos T (1985) Nucleic Acids Res 13:6545–6558
Bird AP (1987) Trends Genet 3:342–347
Broach JR, Li Y-Y, Wu L-C, Jayaram M (1983) In: Inouye (ed) Experimental manipulation of gene expression. Academic Press, New York, pp 83–117
Brooks JE, Blumenthal RM, Gingeras TR (1983) Nucleic Acids Res 11:837–851
Bull JR, Wootton JC (1984) Nature 310:701–704
Cedar H, Solage A, Glaser G, Razin A (1979) Nucleic Acids Res 6:2125–2132
Doerfler W (1983) Annu Rev Biochem 52:93–124
Doerfler W (1984) Curr Top Microbiol Immunol 108:79–98
Fehér Z, Kiss A, Venetianer P (1983) Nature 302:266–268
Fehér Z, Schablik M, Kiss Á, Zsindely A, Szabó G (1986) Curr Genet 11:131–137
Fehér Z, Schlagman SL, Miner Z, Hattman S (1988) Gene 73:193–195
Gorovsky MA, Hattman S, Pleger GL (1973) J Cell Biol 56:697–701
Günthert U, Reiners L (1987) Nucleic Acids Res 15:3689–3702
Higgins DR, Prakash L, Reynolds P, Prakash S (1984) Gene 30:121–128
Hoekstra MF, Malone RE (1985) Mol Cell Biol 5:610–618
Hoekstra MF, Malone RE (1986) Mol Cell Biol 6:3555–3558
Jupe ER, Magill JM, Magill CW (1986) J Bacteriol 165:420–423
Kiss A, Baldauf F (1983) Gene 21:111–119
Miller R, Prakash L, Prakash S (1982) Mol Cell Biol 2:939–948
Miner Z (1989) PhD dissertation, University of Rochester, Rochester, NY, USA
Pósfai G, Baldauf F, Erdei S, Pósfai J, Venetianer P, Kiss A (1984) Nucleic Acids Res 12:9039–9049
Proffitt JH, Davie JR, Swinton D, Hattman S (1984) Mol Cell Biol 4:985–988
Rogers SD, Rogers ME, Saunders G, Holt G (1986) Curr Genet 10:557–560
Russell PJ, Rodland KD, Rachlin EM, McCloskey JA (1987) J Bacteriol 160:2902–2905
Selker EU, Stevens JN (1985) Proc Natl Acad Sci USA 82:8114–8118
Specht CA, Novotny CP, Ullrich RC (1984) Curr Genet 8:219–232
Tamame J, Antequera FM, Villaneuva JR, Santos T (1983) Moll Cell Biol 3:2287–2297
Urieli-Shoval S, Gruenbaum Y, Sedat J, Razin A (1982) FEBS Letts 146:148–152
Vanyushin BF, Kadrova DKh, Karimov KhKh, Belozerskii AN (1971) Biokhimya (English translation) 36:1048–1053
Wilcox DR, Prakash L (1981) J Bacteriol 148:618–623
Zolan ME, Pukkila PJ (1985) UCLA Symp Mol Cell Biol 34:333–344
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Fehér, Z., Schlagman, S.L., Miner, Z. et al. The UV excision-repair system of Saccharomyces cerevisiae is involved in the removal of methylcytosines formed in vivo by a cloned prokaryotic DNA methyltransferase. Curr Genet 16, 461–464 (1989). https://doi.org/10.1007/BF00340726
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DOI: https://doi.org/10.1007/BF00340726