Summary
The saccus vasculosus of rainbow trout and brown trout, the latter caught in the wild, has been investigated by histochemical means. Isolated coronet cells and groups of them were found to be rather strongly but unspecifically stainable by alcian blue. A performic acid-aniline-aldehyde-thionine reaction demonstrated that such cells contain more disulfide groups than their nonstaining neighbours. This higher disulfide content and the stainability by alcian blue do not necessarily coincide with the presence of acid mucopolysaccharide, which was found in the cytoplasm of coronet cells in some cases. The hypothesis is discussed that cystine may be stored and used by coronet cells as a precursor of the acid mucopolysaccharide, which has been shown in the lumen of the organ.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literatur
Adams, C. W. M., and A. G. E. Pearse: Classification of the mucoid (basophil) cells in the normal and pathological human adenohypophysis. J. Endocr. 18, 147–153 (1959).
Bargmann, W.: Über Feinbau und Funktion des Saccus vasculosus. Z. Zellforsch. 40, 49–74 (1954).
Dorn, E.: Der Saccus vasculosus. In: Handbuch der mikroskopischen Anatomie des Menschen, herausgeg von. W. Bargmann, Bd. IV/2. Berlin-Göttingen-Heidelberg: Springer 1955.
Elftman, H.: A chrome-alum fixative for the pituitary. Stain Technol. 32, 25–28 (1957).
Jansen, W. F., and J. C. van de Kamer: Histochemical analysis and cytological investigations on the coronet cells of the saccus vasculosus of the rainbow trout (Salmo irideus). Z. Zellforsch. (im Druck).
Kamer, J. C. Van de, J. Boddingius and J. Boender: On the structure and function of the saccus vasculosus of the rainbow trout (Salmo irideus). Z. Zellforsch. 52, 494–500 (1960).
Lillie, R. D., and G. G. Glenner: Histochemical aldehyde blockade by aniline in glacial acetic acid. J. Histochem. Cytochem. 5, 167–169 (1957).
Mowry, R. W.: Alcian blue technics for the histochemical study of acidic carbohydrates. J. Histochem. Cytochem. 4, 407 (1956).
Paget, G. E., and E. Ecclestone: Aldehyde-thionine: a stain having similar properties to aldehyde-fuchsine. Stain Technol. 34, 223–226 (1959).
—: Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Stain Technol. 35, 119–122 (1960).
Pearse, A. G. E.: Histochemistry, theoretical and applied, 2nd edit. London: J. & A. Churchill 1960.
Romeis, B.: Mikroskopische Technik. München: R. Oldenbourg 1948.
Stahl, A., et R. Seite: Contribution à l'étude du sac vasculaire des poissons téléostéens. C. R. Soc. Biol. (Paris) 154, 1020–1022 (1960).
Young, L., and G. A. Maw: The metabolism of sulphur compounds. London: Methuen 1958.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zwillenberg, L.O. Histochemische Beobachtungen am Saccus vasculosus der Forelle. Zeitschrift für Zellforschung 54, 437–442 (1961). https://doi.org/10.1007/BF00340448
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00340448