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Mechanism of conjugation and recombination in bacteria

IX. The role of DNA synthesis in post-conjugal genetic recombination

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Summary

Development of resistance to 23P-decay of donor genetic determinants after their transfer into the female cell is dependent on unabated DNA synthesis. A similar dependence upon DNA synthesis was found in recombinational events. Both processes show a similar time-course. The DNA synthesis, involved, seems distinct from physiological replication of the chromosome. The formation of the structure resistant to 32P-decay is going on concomitantly with recombinational process and is completed within 45 to 55 minutes after transfer, before beginning of the replication of recombinant structure. The bearing of these facts on the molecular mechanism of genetic recombination is discussed.

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Authors and Affiliations

  1. Department of General Microbiology, Insitute of Microbiology, University of Warsaw, Warsaw, Poland

    Mirosława Włodarczyk & Władysław Kunicki-Goldfinger

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  1. Mirosława Włodarczyk
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  2. Władysław Kunicki-Goldfinger
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Communicated by W. Gajewski

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Włodarczyk, M., Kunicki-Goldfinger, W. Mechanism of conjugation and recombination in bacteria. Molec. Gen. Genetics 106, 263–273 (1970). https://doi.org/10.1007/BF00340385

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  • DOI: https://doi.org/10.1007/BF00340385

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