Summary
The effect of deoxyadenosine (AdR) and 5-fluorodeoxyuridine (FUdR) on the incorporation of 5-bromodeoxyuridine (BUdR) into DNA of thymine-non requiring cells of Escherichia coli was studied. This incorporation renders the cells sensitive to irradiation at near-UV light. It was found that the combination of AdR and FUdR increases incorporation of BUdR and sensitivity to irradiation.
An enrichment of auxotrophs by a factor of 105 was obtained from a mixed population grown in minimal medium containing BUdR, AdR and FUdR and irradiated subsequently.
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Communicated by E. Witkin
Part of an M.Sc. Thesis in Microbiology submitted by A. Rosner to the Tel-Aviv University.
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Rosner, A., Yagil, E. Incorporation of 5-bromodeoxyuridine into DNA of wild type Escherichia coli and its use for the enrichment of auxotrophic mutants. Molec. Gen. Genetics 106, 254–262 (1970). https://doi.org/10.1007/BF00340384
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DOI: https://doi.org/10.1007/BF00340384