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Comparative immunochemical studies with antisera to sheep prolactin and bovine growth hormone on anterior pituitaries of ox, sheep, rats and mice

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Summary

Rabbit antisera to sheep prolactin and bovine growth hormone were used in the indirect fluorescent antibody technique on cryostat sections of anterior pituitaries of sheep, ox, rats and mice. It is demonstrated that in sheep and ox prolactin and growth hormone are manufactured by different acidophilic pituitary cells. Though the antisera do not precipitate the analogous hormones of rats and mice in gel diffusion tests, evidence is given for the specificity of the cross-reaction of the antisera with the analogous murine hormones in situ as found with the fluorescent antibody technique.

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Authors and Affiliations

  1. Department of Immunology, Antoni van Leeuwenhoek-huis, The Netherlands Cancer Institute, Amsterdam, The Netherlands

    Philip Rümke & Nita C. J. J. Ladiges

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  1. Philip Rümke
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  2. Nita C. J. J. Ladiges
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Additional information

We are grateful to Dr. J. D. H. Homan of Organon, Oss (The Netherlands) for the supply of bovine growth hormone and for kindly giving us the information concerning this preparation.

We should like to thank Dr. F. J. A. Prop for the prolactin assays, Dr. H. G. Kwa for providing the mouse pituitary tumours and Dr. L. M. Boot for the mouse pituitary isograft in the kidney, Mrs. J. J. Geiger-Koedijk for the conventional staining of pituitary sections, and Mr. J. van der Kamp for the photography.

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Rümke, P., Ladiges, N.C.J.J. Comparative immunochemical studies with antisera to sheep prolactin and bovine growth hormone on anterior pituitaries of ox, sheep, rats and mice. Zeitschrift für Zellforschung 67, 575–583 (1965). https://doi.org/10.1007/BF00340325

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  • DOI: https://doi.org/10.1007/BF00340325

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