Summary
We have tested for the presence of the receptor for the Escherichia coli phage T4 in different isolates of the plant pathogenic enterobacteria Erwinia carotora subsp. carotovora and subsp. atroseptica. Several of the isolates appeared to contain a functional T4 receptor as shown by phage adsorption and phage-induced lysis of the bacteria. Two of the isolates could even sustain lytic growth of T4. In addition, we show that the transducing derivative of T4, T4GT7, can be employed to transfer plasmids from E. coli to E. carotovora thus opening up new possibilities for genetic analysis of Erwinia.
This is a preview of subscription content, log in via an institution to check access.
References
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Boyer HW, Roulland-Dussoix D (1969) A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41:459–472
Casadaban MJ (1976) Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol 104:541–555
DeBoer SH, Copeman RJ, Vruggink H (1979) Serogroups of Erwinia carotovora potato strains determined with diffusible somatic antigens. Phytopathology 69:316–319
DeBoer SH, Vruggink H, Verdonckt L, Harju P, Bang HO, DeLey J (1987) Serological and biochemical variation among potato strains of Erwinia carotovora subsp. atroseptica and their taxonomic relationship to other E. carotovora strains. Appl Bacteriol 63:487–495
Forbes KJ, Perombelon MCM (1985) Chromosomal mapping in Erwinia carotovora subsp. carotovora with the IncP plasmid R68::Mu. J Bacteriol 164:1110–1116
Hinton JCD, Pérombelon MCM, Salmond GPC (1985a) Efficient transformation of Erwinia carotovora subsp. cartovora and E. carotovora subsp. atroseptica. J Bacteriol 161:786–788
Hinton JCD, Pérombelon MCM, Salmond GPC (1985b) Nonsense-suppressor mutants of Erwinia carotovora subsp. carotovora. FEMS Microbiol Lett 28:103–106
Jayaswal RK, Bressan RA, Handa AK (1984) Mutagenesis of Erwinia carotovora subsp. carotovora with bacteriophage Mud1 (Apr lac cts62): Construction of his-lac gene fusions. J Bacteriol 158:764–766
Korhonen TK, Kalkkinen N, Haahtela K, Old DC (1987) Characterization of type I and mannose-resistant fimbriae of Erwinia spp. J Bacteriol 169:2281–2283
Mindich L, Cohen J, Weisburd M (1976) Isolation of nonsense suppressor mutants in Pseudomonas. J Bacteriol 126:177–182
Palva ET, Harkki A, Karkku H, Long H, Pirhonen M (1987) λ vehicles provide new tools for genetic analysis of Gram-negative bacteria. Microbial Pathogenesis 3:227–230
Pérombelon MCM, Kelman A (1980) Ecology of the soft rot erwinias. Annu Rev Phytopathol 18:361–387
Prehm P, Jann B, Jann K, Schmidt G, Stirm S (1976) On a bacteriophage T3 and T4 receptor region within the cell wall lipopolysaccharide of Escherichia coli B. J Mol Biol 101:277–281
Saarilahti HT, Palva ET (1986) Major outer membrane proteins in the phytopathogenic bacteria Erwinia carotovora subsp. carotovora and subsp. atroseptica. FEMS Microbiol Lett 35:267–270
Salmond GPC, Hinton JCD, Gill DR, Pérombelon MCM (1986) Transposon mutagenesis of Erwinia using phage λ vectors. Mcl Gen Genet 203:524–528
Sandulache R, Prehm P (1985) Structure of the core oligosaccharide from lipopolysaccharide of Erwinia carotovora. J Bacteriol 161:1226–1227
Takahashi H, Saito H (1982a) High-frequency transduction of pBR322 by cytosine-substituted T4 bacteriophage: evidence for encapsulation and transfer of head-to tail plasmid concatemers. Plasmid 8:29–35
Takahashi H, Saito H (1982b) Mechanism of pBR322 transduction mediated by cytosine-substituting T4 bacteriophage. Mol Gen Genet 186:497–500
Wilson GG, Young KKY, Edlin GJ, Konigsberg W (1979) Highfrequency generalized transduction by bacteriophage T4. Nature 280:80–82
Young KKY, Edlin G (1983) Physical and genetic analysis of bacteriophage T4 generalized transduction. Mol Gen Genet 192:241–246
Young KKY, Edlin GJ, Wilson GG (1982) Genetic analysis of bacteriophage T4 transducing bacteriophages. J Virol 41:345–347
Zinck RT, Kemble RJ, Chatterjee AK (1984) Transposon Tn5 mutagenesis in Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica. J Bacteriol 157:809–814
Author information
Authors and Affiliations
Additional information
Communicated by K. Isono
Rights and permissions
About this article
Cite this article
Pirhonen, M., Palva, E.T. Occurrence of bacteriophage T4 receptor in Erwinia carotovora . Mol Gen Genet 214, 170–172 (1988). https://doi.org/10.1007/BF00340198
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00340198