Summary
MATa cells of Saccharomyces cerevisiae defective in both the SST1 and SST2 gene products exhibit selfarrest when they express the MFα1 gene under the control of the GAL1 promoter. This reponse to endogenously produced pheromone can be alleviated by mutations which prevent the production of, or response to, α-factor. Suppressors of the self-arrest phenotype include a class of mutants which remain responsive to low levels of pheromone, but are resistant to high levels of α-factor. One of these mutants has been mapped to chromosome X, 31 cM distal to SUP4, and defines a new locus designated STE18.
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Communicated by C.P. Hollenberg
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Whiteway, M., Hougan, L. & Thomas, D.Y. Expression of MFα1 in MATa cells supersensitive to α-factor leads to self-arrest. Mol Gen Genet 214, 85–88 (1988). https://doi.org/10.1007/BF00340184
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DOI: https://doi.org/10.1007/BF00340184