Summary
In Bufo bufo bufo larvae treated with Ca45 prior to the onset of the mineralization of the skeletal pieces, fixed after 7 days treatment (early hind-limb bud stage), the greatest amount of Ca45 is detectable in the lumens of the endolymphatic sacs. A small amount of Ca45 is concentrated in the membranous labyrinth. By the end of metamorphosis the lumina of the endolymphatic sacs are only partially occupied by Ca45CO3 crystals. On the other hand Ca45 localization is evidenced in the epithelium lining the sacs and in the developing bones. The present observations seem to afford a demonstration that the Ca salts of the endolymphatic sacs take part in the ossification process.
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The present investigations have been supported by grants of the Consiglio Nazionale delle Ricerche and the University of Torino to the Institute of Comparative Anatomy and Histology.
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Guardabassi, A. The utilization of the calcareous deposits of the endolymphatic sacs of Bufo Bufo Bufo in the mineralization of the skeleton. Investigations by means of Ca45 . Zeitschrift für Zellforschung 51, 278–282 (1960). https://doi.org/10.1007/BF00339969
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DOI: https://doi.org/10.1007/BF00339969