Summary
Synapses were found in rat cerebellar and brainstem cultures with the electron microscope. Three distinct types of synaptic terminals were described. The similarity between synapses found in vitro and in vivo was emphasized.
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Bunge, R., M. Bunge, E. Peterson, and M. Murray: Ultrastructural similarities between in vitro and in vivo central nervous tissue. Abstracts Third Annual Meeting American Society for Sell Biology. J. Cell Biol. 19, 11 a (1963).
De Robertis, E.: Submicroscopic morphology and function of the synapse. Exp. Cell Res., Suppl. 5, 347–369 (1958).
De Robertis, E., and H. S. Bennett: Some features of the submicroscopic morphology of synapses in frog and earthworm. J. biophys. biochem. Cytol. 1. 47–58 (1955).
Gray, E. G.: Electron microscopy of synaptic organelles of the central nervous system. Internat. Congr. of Neuropathology. II. Thema II and III, p. 57–61 (1961).
Hild, W.: Myelogenesis in cultures of mammalian central nervous tissue. Z. Zellforsch. 46, 71–95 (1957).
—, and I. Tasaki: Morphological and physiological properties of neurons and glial cells in tissue culture. J. Neurophysiol. 25, 277–304 (1962).
Levi, G.: Explantation, besonders die Struktur und die biologischen Eigenschaften der in vitro gezüchteten Zellen und Gewebe. Ergebn. Anat. Entwickl.-Gesch. 31, 125–608 (1934).
Palay, S. L.: The morphology of synapses in the central nervous system. Exp. Cell Res., Suppl. 5, 275–293 (1958).
Peterson, E. R., and M. R. Murray: Myelin sheath formation in cultures of avian spinal ganglia. Amer. J. Anat. 96, 319–356 (1955).
Whittaker, V. P., and E. G. Gray: The synapse: Biology and morphology. Brit. med. Bull. 18, 223–228 (1962).
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Supported by USPHS Grants 5 Tl 459-04 and NB 03114-03S1 from the National Institutes of Health, Bethesda, Maryland.
The authors wish to express their sincere appreciation to Mrs. Eleanor Morris for her assistance in preparing the cultures and Mr. Earl Pitsinger for his photographic assistance.
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Callas, G., Hild, W. Electron microscopic observations of synaptic endings in cultures of mammalian central nervous tissue. Zeitschrift für Zellforschung 63, 686–691 (1964). https://doi.org/10.1007/BF00339915
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DOI: https://doi.org/10.1007/BF00339915