Summary
Two days cultures of HeLa cells were stimulated for a short time with ATP and incubated in a solution of ferritin. The localization of the marker was studied after 15 min. The ferritin particles were encountered in smooth surfaced vacuoles, in the Golgi cisternae and vesicles, in dense bodies, multivesicular bodies and crystal-containing bodies. Ferritin was never observed in the nucleus, for this time of incubation. These observations are discussed in comparison with results obtained after an incubation in 3H-DNA.
Similar content being viewed by others
References
Ahearn, M., T. Hamilton, and J. Biesele: Serum-induced formation of lysosomes in HeLa cells: a process sensitive to actinomycin D. Proc. nat. Acad. Sci. (Wash.) 55, 852–857 (1966).
Bensch, K., G. Gordon, and L. Miller: The fate of DNA-containing particles phagocytized by mammalian cells. J. Cell Biol. 21, 105–114 (1964).
Blondel, B., and L. Tolmach: Three phases of the HeLa Cell cycle. Exp. Cell Res. 37, 497–501 (1965).
Brightman, M.: The distribution within the brain of ferritin injected into cerebrospinal fluid compartments. I. Ependymal distribution. J. Cell Biol. 26, 99–123 (1965).
Driessens, J., A. Dupont, A. Demaille, P. Cappelaere, E. Puvion et Y. Herbin: Etude cytologique au microscope électronique de la cellule HeLa. C.R. Soc. Biol. (Paris) 159, 843–845 (1965).
Epstein, M.: Some unusual features of fine structure observed in HeLa cells. J. biophys. biochem. Cytol. 10, 153–162 (1961).
Feldherr, C.: The intracellular distribution of ferritin following microinjection. J. Cell Biol. 12, 159–167 (1962).
George, P., L. Journey, and M. Goldstein: Effect of vincristine on the fine structure of HeLa cells during mitosis. J. nat. Cancer Inst. 35, 355–375 (1965).
Gordon, G., L. Miller, and K. Bensch: Studies on the intracellular digestive process in mammalian tissue culture cells. J. Cell Biol. 25, 41–55 (1965).
Gropp, A.: Phagocytosis and pinocytosis. in: Cinemicrography in cell biology (ed. G. G. Rose), p. 279–312. Academic Press 1963.
Harford, C., A. Hamlin, and E. Parker: Electron microscopy of HeLa cells after the ingestion of colloidal gold. J. biophys. biochem. Cytol. 3, 749–756 (1957).
Loni, M. C.: Effet de l'ATP sur l'incorporation d'un DNA-H3 exogène dans les cellules HeLa en culture. Experientia (Basel) 22, 711–712, (1966).
Morgan, C., G. Godman, P. Breitenfeld, and A. Rose: A correlative study by electron and light microscopy of the development of type 5 adenovirus. I. Electron microscopy. J. exp. Med. 112, 373–382 (1960).
—, H. Rose, M. Holden, and E. Jones: Electron microscopic observations on the development of Herpes simplex virus. J. exp. Med. 110, 643–656 (1959).
Policard, A., and M. Bessis: Micropinocytosis and rhopheocytosis. Nature (Lond.) 194, 110–111 (1962).
Robbins, E., and N. Gonatas: The ultrastructure of a mammalian cell during the mitotic cycle. J. Cell Biol. 21, 429–463 (1964a).
—: Histochemical and ultrastructural studies on HeLa cells cultures exposed to spindle inhibitors with special reference to the interphase cell. J. Histochem. Cytochem. 12, 704–711 (1964b).
Rose, L. C.: Mikrokinetospheres and VP satellites of pinocytic cells observed in tissue cultures of Gey's strain HeLa with phase contrast cinematographic techniques. J. biophys. biochem. Cytol. 3, 697–703 (1957).
Ryser, H., J. Caulfield, and J. Aub: Studies on protein uptake by isolated tumor cells. I. Electron microscopic evidence of ferritin uptake by Ehrlich ascites tumor cells. J. Cell Biol. 14, 255–268 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Loni, M.C., Borgers, M. & Hugon, J. Uptake of ferritin particles by ATP-stimulated hela cells. Zeitschrift für Zellforschung 76, 525–531 (1967). https://doi.org/10.1007/BF00339753
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00339753