Summary
Adaptations of currently available autoradiographic electron microscopic methods to the study of organelle development in single Paramecium cells are described. Cells of known age were pulse-labeled with tritiated leucine and observed either as whole mounts, or as sections of specified age and cell-lineage. Labeled trichocysts appear as early as one hour after uptake and retain the label without dilution for at least 3 fission generations thereafter. Cells grown in unlabeled medium, but derived from labeled cells, show the bulk of the residual label to be conservatively associated with the stable structural organelles of the cell surface. These organelles include those trichocysts and ciliary corpuscles that had been synthesized in the ancestral progenitor cell during or shortly after administration of the isotopically labeled amino acid.
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Dedicated to Professor Friedrich Wassermann with admiration and affection on the occassion of his eightieth birthday.
Work supported by U. S. Atomic Energy Commission.
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Ehret, C., Savage, N. & Alblinger, J. Patterns of segregation of structural elements during cell division. Z.Zellforsch 64, 129–139 (1964). https://doi.org/10.1007/BF00339192
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DOI: https://doi.org/10.1007/BF00339192