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Harvesting and culture of epithelial cells from hollow organs of the female reproductive system

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Summary

The technique of trypsinization of cells lining the hollow organs applied to the female reproductive tract has proven successful in harvesting living cells which produced luxuriant cultures in vitro.

Elements from the oviduct, endometrial cavity and vagina were obtained from 6 rabbits, 2 dogs and 6 surgical specimens by means of bathing the lumen of the organs with the use of a 0.5% solution of trypsin in calcium and magnesium free Gey's balanced salt solution for 30 to 40 min, employing continued gentle agitation provided by a syringe.

Epithelial cells so harvested were grown in Rose chambers and in T-30 flasks for as long as 30 days, when they were fixed for study with special stains.

No fibrocytic contamination of the cultures was observed.

Further applications of the method are under investigation and are briefly outlined in the discussion.

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References

  • Cron, R. S., and G. O. Gey: The viability of the cast-off menstrual endometrium. Amer. J. Obstet. Gynec. 13, 645 (1927).

    Google Scholar 

  • Hirsch, E. F., and H. O. Jones: The behaviour of the epithelium in explants of human endometrium. Amer. J. Obstet. Gynec. 25, 37 (1933).

    Google Scholar 

  • Maruyama, Y.: Human endothelial cells in tissue culture. Z. Zellforsch. (in press).

  • Moore, J. G.: Growth characteristics of normal and malignant cervical epithelium in tissue culture. Amer. J. Obstet. Gynec. 64, 13 (1952).

    Google Scholar 

  • —: Growth characteristics in tissue culture of controversial lesions of the uterine cervix. West. J. Surg. 63, 1 (1955).

    Google Scholar 

  • —: Tissue-culture studies of endometrium explanted at varying stages of the menstrual cycle. Fertil and Steril. 7, 411 (1956).

    Google Scholar 

  • Pomerat, C. M., and W. C. Slick: Isolation and growth of endothelial cells in tissue culture. Nature (Lond.) (in press).

  • Rinaldini, L. M. J.: The isolation of living cells from animal tissues. In: Internat. Rev. Cytol., vol. VII, p. 587. New York: Academic Press 1958.

    Google Scholar 

  • Traut, H. F.: Adult human endometrium in tissue culture. Surg. Gynec. Obstet. 47, 334 (1928).

    Google Scholar 

  • Valle, J. R., and C. M. Pomerat: Cultivation of endometrium in vitro-a preliminary note. Tex. Rep. Biol. Med. 5, 145 (1947).

    Google Scholar 

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, State University of New York, Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, New York

    Carlo Valenti

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  1. Carlo Valenti
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Additional information

This work was performed in the Department of Cellular Biology, Pasadena Foundation for Medical Research, Pasadena, California, and aided in part by a grant from the U. S. Public Health Service, No. 2 G 279 and from the U. S. Army Medical Research and Development Command, Department of the Army, under Research Grant No. DA-MD-49-193-63-G80 administered by C. M. Pomerat.

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Valenti, C. Harvesting and culture of epithelial cells from hollow organs of the female reproductive system. Zeitschrift für Zellforschung 60, 850–859 (1963). https://doi.org/10.1007/BF00339096

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  • DOI: https://doi.org/10.1007/BF00339096

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