Summary
In the endothelial-like elements dissociated from rat heart tissue and maintained in vitro, approximately 10% demonstrated evaginations or blebs of the nuclear membrane at any moment in time. Analyses of time-lapse cinematographic records revealed that nearly 60% of the cell population underwent extrusion of nuclear material prior to mitosis. Cytochemical studies showed that these blebs were divided into a stainable area and a clear zone. The constituents of the latter portion were negative to staining with Feulgen reaction, hematoxylin, methylene blue, acridine orange, sudan black, and May-Grünwald-Giemsa. In addition, cultures treated with either tritiated thymidine or uridine failed to demonstrate a tag in this area. Although the constituents of the clear zone were considered to represent the bulk of the extruded material, the high frequency of the nucleolar association with the adjacent stainable area suggested the possibility of a transfer of nucleolar components to the cytoplasm. A second potential function of nuclear blebbing, as related to cytoplasmic membrane formation, is discussed.
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Aided by Grant GB-15 from the National Science Foundation, administered by Dr. C. M. Pomerat.
Grateful thanks are extended to Drs. C. M. Pomerat and D. E. Rounds who provided valuable suggestions and encouragement throughout the study. Mr. Charles Raiborn rendered indispensable aid with the various culture procedures employed. Credit is due Messrs. C. George Lefeber and Robert Olson for their generous assistance with photographic technique. Mrs. Paul Melnychyn contributed notably in the preparation of the manuscript.
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Kyte, J. Nuclear extrusion in dissociated rat heart cells. Zeitschrift für Zellforschung 62, 495–503 (1964). https://doi.org/10.1007/BF00338845
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DOI: https://doi.org/10.1007/BF00338845