Summary
The turnover ofl-5-HTP,d-5-HTP and 5-HT in the exocrine pancreas have been studied by means of the fluorescence method ofFalck andHillarp. l- andd-5-HTP are easily taken up by the acinar cells, whereas 5-HT seems to pass into the cells only to a minor extent. After the administration ofl-5-HTP (and in some cases after 5-HT administration), specific fluorescence is seen in the form of apically located granules (probably identical with the zymogen granules) for a short period, which is prolonged, if the animals are pretreated with a MAO inhibitor. Decarboxylase inhibition prevents the appearance of these fluorescent granules. Administration ofd-5-HTP does not give rise to this granular fluorescence but to a diffuse fluorescence throughout the cells. Thus, there are reasons to assume that the granular fluorescence derives from 5-HT. The results obtained in this work correspond well with those from a similar study withl-DOPA and some of its analogues.
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Abbreviations
- DOPA:
-
3,4-dihydroxyphenylalanine
- DA:
-
dopamine
- NA:
-
noradrenaline
- A:
-
adrenaline
- 5-HTP:
-
5-hydroxytryptophan
- 5-HT:
-
5-hydroxytryptamine
- MAO:
-
monoamine oxidase
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This work was supported by grants from the Swedish Medical Research Council (B68-12X-712-03B and B68-14X-56-04B), the United States Public Health Service (06701-02) and the Faculty of Medicine, University of Lund, Lund, Sweden.
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Alm, P. Fluorescence microscopy of the 5-HTP turnover in the exocrine pancreas of mice and rats. Z. Zellforsch. 96, 212–221 (1969). https://doi.org/10.1007/BF00338768
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DOI: https://doi.org/10.1007/BF00338768