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Kinetics of Mu DNA synthesis

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Summary

Mu specific DNA synthesis starts at 10 min after infection. All essential amber mutants of Mu were tested for the ability to replicate in a non permissive host. Except for the amber mutants A and B, which were already known to be blocked in Mu DNA synthesis (Wijffelman et al., 1974), all the other mutants showed normal Mu DNA replication.

Using mitomycin C-treated cells Mu DNA synthesis was found to start at about 20 min after induction. However using the much more sensitive method of DNA-RNA hybridization, it was found that the DNA synthesis starts already at 10 min after induction, and that at 20 min after induction about 7 copies of the Mu DNA are present per cell.

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References

  • Bukhari, A., Zipser, D.: Random insertion of Mu-1 DNA within a single gene. Nature (Lond.) New Biol. 236, 240–243 (1972)

    Google Scholar 

  • Burton, K.: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62, 315–323 (1956)

    Google Scholar 

  • Faelen, M., Toussaint, A.: Isolation of conditional defective mutants of temperate phage Mu-1 and deletion mapping of the Mu-1 prophage. Virology 54, 117–124 (1973)

    Google Scholar 

  • Giles, K., Myers, A.: An improved Diphenylamine method for the Estimation of Deoxyribonucleic acid. Nature (Lond.) 206, 93 (1965)

    Google Scholar 

  • Glickman, B.W., Walker, K.J., Rörsch, A.: The repair of 32P decay damaged phage λ in various radiation-sensitive strains of E. coli K12. Mutation Res. 14, 265–270 (1972)

    Google Scholar 

  • Hsu, M., Davidson, M.: Structure of inserted bacteriophage Mu-1 DNA and physical mapping of bacterial genes by Mu-1 DNA insertion. Proc. nat. Acad. Sci. (Wash.) 69, 2823–2827 (1972)

    Google Scholar 

  • Lindquist, B.: Vegetative DNA of temperate coliphage P2. Molec. gen. Genet. 110, 178–196 (1971)

    Google Scholar 

  • Lindquist, B., Sinsheimer, R.: The process of infection with bacteriophage ΦX174. XV Bacteriophage DNA synthesis in abortive infection with a set of conditional lethal mutants. J. molec. Biol. 30, 69–80 (1967)

    Google Scholar 

  • Putte, P. van de, Gruijthuijsen, M.: Chromosome-mobilization and integration of F-factors in the chromosome of recA strains of E. coli under the influence of bacteriophage Mu-1. Molec. gen. Genet. 118, 173–183 (1972)

    Google Scholar 

  • Schroeder, W., Bade, E., Delius, H.: Participation of Escherichia coli DNA in the replication of temperate bacteriophage Mu-1. Virology 60, 534–542 (1974)

    Google Scholar 

  • Stevens, W., Adhya, S., Szybalski, W.: Origin and bidirectional orientation of DNA. Replication in coliphage lambda. In: The bacteriophage lambda (Hershey, A., ed.). Cold Springer Harbor Laboratory 1971

  • Taylor, A.: Bacteriophage-induced mutation in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 50, 1043–1051 (1963)

    Google Scholar 

  • Toussaint, A., Faelen, M.: Connecting two unrelated DNA sequences with a Mu dimer. Nature (Lond.) New Biol. 242, 1–4 (1973)

    Google Scholar 

  • Vogel, H., Bonner, D.: Acetylornithase of Escherichia coli: partial purification and some properties. J. biol. Chem. 218, 97–106 (1956)

    Google Scholar 

  • Waggoner, B.T., Gonzalez, N., Taylor, A.L.: Isolation of heterogeneous circular DNA from induced lysogens of bacteriophage Mu-1. Proc. nat. Acad. Sci. (Wash.) 71, 1255–1259 (1974)

    Google Scholar 

  • Wijffelman, C., Gassler, M., Stevens, W., Putte, P. van de: On the control of transcription of bacteriophage Mu. Molec. gen. Genet. 131, 85–96 (1974)

    Google Scholar 

  • Wijffelman, C., Putte, P. van de: Transcription of bacteriophage Mu. An analysis of the transcription pattern in the early phase of phage development. Molec. gen. Genet. 135, 327–337 (1974)

    Google Scholar 

  • Wijffelman, C., Westmaas, G., Putte, P. van de: Vegetative recombination of bacteriophage Mu-1. Molec. gen. Genet. 116, 40–46 (1972)

    Google Scholar 

  • Wijffelman, C., Westmaas, G., Putte, P. van de: Similarity of the vegetative and prophage map of bacteriophage Mu-1. Virology 54, 125–134 (1973)

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Genetics, State University, Leiden, The Netherlands

    Carel Wijffelman

  2. Laboratory of Molecular Genetics, State University, Leiden, The Netherlands

    Bep Lotterman

Authors
  1. Carel Wijffelman
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  2. Bep Lotterman
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Communicated by G. Bertani

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Wijffelman, C., Lotterman, B. Kinetics of Mu DNA synthesis. Molec. gen. Genet. 151, 169–174 (1977). https://doi.org/10.1007/BF00338691

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  • DOI: https://doi.org/10.1007/BF00338691

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