Summary
A factor which promotes growth of diploid fibroblasts and amniotic fluid cells was prepared from human urine. The addition of up to 10% of this growth factor together with 20% fetal calf serum to the culture medium was found to be optimal for stimulation of amniotic fluid cell growth. Microtest plates have several advantages compared with other methods to initiate an amniotic cell culture.
Zusammenfassung
Das Wachstum von Amnionzellen und diploiden Fibroblasten wird durch Konzentrat von menschlichem Urin stimuliert. Ein optimales Anwachsen von Amnionzellen wurde erreicht, wenn das Kulturmedium mit 20% fetalem Kälberserum und bis zu 10% Urinkonzentrat versetzt wurde. Das Anlegen einer Amnionzellkultur in Mikrotestplatten hat Vorteile gegenüber anderen Methoden.
References
Dorfman, A., Ed.: Antenatal diagnosis. Chicago: Univ. of Chicago Press 1972
Dulbecco, R.: Topoinhibition and serum requirements of transformed and untransformed cells. Nature (Lond.) 227, 802 (1970)
Emery, A. E. H., Ed.: Antenatal diagnosis of genetic diseases. Edinburgh: Churchill Livingstone 1972
Gail, M. H., Brone, C. W.: Density inhibition of motility in 3T3 fibroblasts and their SV 40 transformants. Exp. Cell Res. 64, 156 (1971)
Holley, R. W., Kiernans, J. A.: Contact inhibition of cell division in 3T3 cells. Proc. nat. Acad. Sci. (Wash.) 60, 300 (1968)
Knörr-Gärtner, H., Härle, I.: A modified method for culturing human amniotic fluid cells for prenatal detection of genetic disorders. Humangenetik 14, 333 (1972)
Lipton, A., Klinger, I., Paul, D., Holley, R. W.: Migration of mouse 3T3 fibroblasts in response to a serum factor. Proc. nat. Acad. Sci. (Wash.) 68, 2799 (1971)
Lipton, A., Paul, D., Henahan, M., Klinger, I., Holley, R. W.: Serum requirements for survival of 3T3 and SV 3T3 fibroblasts. Exp. Cell Res. 74, 466 (1972)
Lüberman, I., Lamy, F., Ore, P.: Nonidentity of fetuin and protein growth (flattening) factor. Science 129, 43 (1959)
Milunsky, A.: The prenatal diagnosis of hereditary disorders. Springfield (Ill.) Thomas 1973
Nadler, H.: Prenatal detection of genetic disorders. In: Advances in human genetics, Vol. 3. Harris, H., Hirschhorn, K., Eds., p. 1. New York-London: Plenum Press 1972
Paul, D., Lipton, A., Klinger, I.: Serum factor requirements for normal and SV 40 transformed 3T3 mouse fibroblasts. Proc. nat. Acad. Sci. (Wash.) 68, 645 (1971).
Pedersen, K. O.: Fetuin, a new globulin isolated from serum. Nature (Lond.) 154, 575 (1944)
Roehm, H., Lipton, A.: Depletion of serum growth factors by 3T3 mouse fibroblasts and vival transformants. Nature (Lond.) New Biol. 245, 115 (1973)
Wolf, C., Lipton, A.: Studies on serum stimulation of mouse fibroblast migration. Exp. Cell Res. 80, 499 (1973)
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Rüdiger, H.W., Wolff, R., Wendel, U. et al. Enhancement of amniotic fluid cell growth in culture. Humangenetik 22, 81–84 (1974). https://doi.org/10.1007/BF00338139
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DOI: https://doi.org/10.1007/BF00338139