Summary
A factor which promotes growth of diploid fibroblasts and amniotic fluid cells was prepared from human urine. The addition of up to 10% of this growth factor together with 20% fetal calf serum to the culture medium was found to be optimal for stimulation of amniotic fluid cell growth. Microtest plates have several advantages compared with other methods to initiate an amniotic cell culture.
Zusammenfassung
Das Wachstum von Amnionzellen und diploiden Fibroblasten wird durch Konzentrat von menschlichem Urin stimuliert. Ein optimales Anwachsen von Amnionzellen wurde erreicht, wenn das Kulturmedium mit 20% fetalem Kälberserum und bis zu 10% Urinkonzentrat versetzt wurde. Das Anlegen einer Amnionzellkultur in Mikrotestplatten hat Vorteile gegenüber anderen Methoden.
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Rüdiger, H.W., Wolff, R., Wendel, U. et al. Enhancement of amniotic fluid cell growth in culture. Humangenetik 22, 81–84 (1974). https://doi.org/10.1007/BF00338139
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DOI: https://doi.org/10.1007/BF00338139