Summary
In addition to the regulator gene C of temperate phage 16-3 of Rhizobium meliloti 41, a second repressor function, called immune X, was identified and cloned into the low copy number cosmid vector pLAFR1. Both repressor functions are necessary to establish complete immunity against superinfecting non-virulent 16-3 strains, but either of the two alone decreases the efficiency of plating (e.o.p.) dramatically. It was shown that the primary target of gene product immune X was the avirT operator locus. The coding region of immune X was localized to the left arm of the phage genome, inside the EcoRI L and H fragments. This region maps about 14 kb from cistron C and had been thought to be genetically silent.
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References
Dallmann G, Orosz L, Sain B (1979) Restriction mapping of DNA of temperate Rhizobium meliloti phage 16-3: comparison of genetic and physical maps indicates a long, genetically silent chromosomal arm. Mol Gen Genet 176:439–448
Dallmann G, Olasz F, Orosz L (1980) Virulent mutants of temperate Rhizobium meliloti phage 16-3 Loci avirC and avirT, and increased recombination. Mol Gen Genet 178:443–446
Dhaese P, Dobbelaera MR, Van Montagu M (1985) The temperate B. subtilis phage φ 105 genome contains at least two distinct regions encoding superinfection immunity. Mol Gen Genet 200:490–492
Dorgai L, Olasz F, Berényi M, Dallmann G, Páy A, Orosz L (1981) Orientation of the genetic and physical map of Rhizobium meliloti temperate phage 16-3. Mol Gen Genet 182:321–325
Dudás B, Orosz L (1980) Correlation between map position and phenotype of Cti mutants in the C cistron of Rhizobium meliloti phage 16-3. Genetics 96:321–329
Friedman, A, Long S, Brown S, Buikema W, Ausubel F (1982) Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene 18:289–296
Orosz L, Sik T (1970) Genetic mapping of Rhizobiophage 16-3, Acta Microbiol Acad Sci Hung 17:185–194
Orosz L (1980) Methods for analysis of the C cistron of temperate phage 16-3 of Rhizobium meliloti. Genetics 94:265–276
Orosz L, Rostás K, Hotchkiss RD (1980) Comparison of two point, three point and deletion mapping in the C cistron of Rhizobiophage 16-3, with an explanation for the recombination pattern. Genetics 94:294–263
Olasz F, Dorgai L, Papp P, Hermesz E, Kósa E, Orosz L (1985) On the site specific recombination of phage 16-3 of Rhizobium meliloti: identification of genetic elements and att recombinations. Mol Gen Genet, in press
Susskind MM, Youderian P (1983) Bacteriophage P22 antirepressor and its control. In: Hendrix RW, Roberts JW, Stahl FW, Weisberg RA (eds) Lambda II. Cold Spring Harbor Laboratory, New York, p 347
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Communicated by J. Schell
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Dorgai, L., Olasz, F. & Németh, K. Lysogenic control of temperate phage 16-3 of Rhizobium meliloti 41 is governed by two distinct regions. Mol Gen Genet 205, 568–571 (1986). https://doi.org/10.1007/BF00338101
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DOI: https://doi.org/10.1007/BF00338101