Summary
A method is described which allows transformation of bacterial cells on the surface of agar plates. It is suitable for transferring large numbers of different plasmids, such as gene libraries, into a new genetic background. One nanogram of plasmid DNA is sufficient for transformation.
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References
Bullas LR, Ryu J-I (1983) Salmonella typhimurium LT2 strains which are r− m+ for all three chromosomally located systems of DNA restriction and modification. J Bacteriol 156:471–474
Cohen SN, Chang AC, Hsu L (1972) Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci USA 69:2110–2114
Lederberg EM, Cohen SN (1974) Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol 119:1072–1074
Schmidt C, Schmieger H (1984) Selective transduction of recombinant plasmids with cloned pac sites by Salmonella phage P22. Mol Gen Genet 196:123–128
Sutcliffe JG (1979) Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp Quant Biol 43:77–90
Vieira J, Messing J (1982) The pUC plasmid, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
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Communicated by G.R. Smith
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Vogel, W., Schmieger, H. Spot-transformation with plasmids. Mol Gen Genet 205, 561–562 (1986). https://doi.org/10.1007/BF00338099
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DOI: https://doi.org/10.1007/BF00338099