Summary
To investigate the interaction of subunits A and B of DNA gyrase during DNA supercoiling, a Cour mutant of Escherichia coli was obtained and the effect of nalidixic acid on the supercoiling of DNA by wild-type and mutant enzymes was assayed. The enzyme of the Cour strain proved to be more sensitive to nalidixic acid than the wild-type DNA gyrase. Hence the mutation affecting the B subunit can also change the properties of the A subunit, which fact suggests that the two subunits of DNA gyrase are in contact during DNA supercoiling.
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Communicated by D. Goldfarb
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Bogdanova, E.S., Mirkin, S.M. & Shmerling, Z.G. Changed properties of the A subunit in DNA gyrase with a B subunit mutation. Mol Gen Genet 186, 572–574 (1982). https://doi.org/10.1007/BF00337967
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DOI: https://doi.org/10.1007/BF00337967