Summary
The incompatibility properties of the bacteriocinogenic plasmid Clo DF13 have been examined. By using Clo DF13, Clo DF13 deletion, and transposon insertion mutants as well as compatible R plasmids into which Clo DF13 fragments have been cloned, we could identify and localise four different incompatibility regions on the Clo DF13 genome. These regions, designated incA, incB, incC, and incD are located in the following positions: incA about incD between 1.8% and 9% of the Clo DF13 genome. We studied the contribution of each of the four inc regions, separately and/or in combination with each other, to the incompatibility between two plasmid replicons. Two types of incompatibility can be distinguished: Type I evoked by incD, that overlaps the replication control area of Clo DF13 and type II, caused by incA, B and C. From our observations we present a model for plasmid incompatibility based on a combination of the existing repressor dilution and membrane attachment models.
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References
Adler HI, Fisher WD, Cohen A, Hardigree AA (1967) Miniature Escherichia coli cells deficient in DNA. Proc Natl Acad Sci USA 57:321–326
Andreoli PM, Overbeeke N, Veltkamp E, Embden JDA van, Nijkamp HJJ (1978) Genetic map of the bacteriocinogenic plasmid Clo DF13 derived by insertion of the transposon Tn901. Mol Gen Genet 160:1–11
Elzen PJM van den, Konings RNH, Veltkamp E, Nijkamp HJJ (1980) Transcription of bacteriocinogenic plasmid Clo DF13 in vivo and in vitro: Structure of the cloacin immunity operon. J Bacteriol 144:579–591
Elzen PJM van den, Maat J, Walters HHB, Veltkamp E, Nijkamp HJJ (1982) The nucleotide sequence of the bacteriocin promoters of plasmids Clo DF13 and Col EI: Role of lex A repressor and c AMP in the regulation of promoter activity. Nucl Acids Res 10:1913–1927
Embden JDA van, (1978) Translocation of an ampicillin resistance determinant within an R-factor aggregate in Salmonella panama. Ant v Leeuwenhoek 44:203–218
Embden JDA van, Veltkamp E, Stuitje AR, Andreoli PM, Njjkamp HJJ (1978) Integration of a transposable DNA sequence which mediates ampicillin resistance into Clo DF13 plasmid DNA: Determination of the site and orientation of Tn901 insertions. Plasmid 1:204–217
Enger-Valk BE, Rotterdam J van, Pouwels PH (1981) The construction of new vehicles for the cloning of transcription termination signals. Nucl Acids Res 9:1973–1989
Hall JD, and Howard-Flanders P (1975) Temperature-sensitive recA Mutant of Escherichia coli K-12: Deoxyribonucleic acid Metabolism after ultraviolet irradiation. J Bacteriol 121:892–900
Hakkaart MJJ, Veltkamp E, Nijkamp HJJ (1981) Protein H encoded by plasmid Clo DF13 involved in lysis of the bacterial host. I Localisation of the gene and identification and subcellular localisation of the gene H product. Mol Gen Genet 183:318–325
Hashimoto-Gotoh, T, Inselburg J (1979) Col E1 plasmid incompatibility: localization and analysis of mutations affecting the incompatibility. J Bacteriol 139:608–619
Hashimoto-Gotoh T, Timmis KN (1981) Incompatibility properties of Col E1 and pMB1 derivative plasmids: random replication of multicopy replicons. Cell 23:229–238
Ishii K, Hashimoto-Gotoh T, Matsubara K (1978) Random replication and random assortment model for plasmid incompatibility in bacteria. Plasmid 1:435–445
Jacob F, Brenner S, Cuzin F (1963) On the regulation of DNA replication in bacteria. CSH Symp Quant Biol 28:329–348
Kool AJ, Nijkamp HJJ (1974) Isolation and characterization of a copy mutant of the bacteriocinogenic plasmid Clo DF13. J Bacteriol 120:569–578
Kostriken R, Morita C, Heffron F (1981) Transposon Tn3 encodes a sitespecific recombination system: Identification of essential sequences, genes, and actual site of recombination. Proc Natl Acad Sci USA 78:4041–4045
Lacatena RM, Cesareni G (1981) Base pairing of RNA I with its complementary sequence in the primer precursor inhibits Col E1 replication. Proc Natl Acad Sci USA 294:623–626
Molin S, Nordström K (1980) Control of plasmid R1 replication: Functions involved in replication, copy number control, incompatibility and switch-off replication. J Bacteriol 141:111–120
Muesing M, Tamm J, Shepard HM, Polinski B (1981) A single base-pair alteration is responsible for the DNA overproduction phenotype of a plasmid copy-number mutant. Cell 24:235–242
Nordström K, Molin S, Aagaard-Hansen H (1980) Partitioning of plasmid R1 in Escherichia coli. I. Kinetics of loss of plasmid derivatives deleted of the par region. Plasmid 4:215–227
Novick RP, Hoppensteadt FC (1978) On plasmid incompatibility. Plasmid 1:421–434
Pol H van de, Veltkamp E, Nijkamp HJJ (1980) Genetic information of the bacteriocinogenic plasmid Clo DF13 involved in the inhibition of the multiplication of double stranded DNA phages. Mol Gen Genet 178:535–540
Pol H van de, Veltkamp E, Nijkamp HJJ (1978) Genetic analysis of the mobilization of the non-conjugative plasmid Clo DF13. Mol Gen Genet 160:139–149
Pritchard RH, Bearth PT, Collins J (1969) Control of DNA synthesis in bacteria. Symp Soc Gen Microbiol 19:263–297
Pritchard RH, Chandler MG, and Collins J (1975) Independence of F replication and chromosome replication in Escherichia coli. Mol Gen Genet 138:143–155
Reed RR, and Grindley NDF (1981) Transposon-mediated sitespecific recombination in vitro: DNA cleavage and protein-DNA linkage at the recombination site. Cell 25:721–728
Staudenbauer WL (1978) Structure and replication of the colicin E1 plasmid. Curr Topics Microbiol Immunol 83:93–157
Stuitje AR, Veltkamp E, Elzen PJM van den, Nijkamp HJJ (1978) In vitro construction of deletion mutants of the bacteriocinogenic plasmid Clo DF13. Nucl Acids Res 5:1801–1820
Stuitje AR, Veltkamp E, Maat J, Heyneker HL (1980) The nucleotide sequence surrounding the replication origin of the cop3 mutant of the bacteriocinogenic plasmid Clo DF13. Nucl Acids Res 8:1459–1473
Stuitje AR, Spelt CE, Veltkamp E, Nijkamp HJJ (1981) Identification of mutations affecting replication control of plasmid Clo DF13. Nature 290:264–267
Tieze GA, Stouthamer AH, Jansz HS, Zandberg J, Bruggen EEJ van (1969) A bacteriocinogenic factor of Enterobacter cloacae. Mol Gen Genet 106:48–65
Timmis KN (1979) Mechanisms of plasmid incompatibility. In: Timmis, KN, Pühler H (eds) Plasmids of medical environmental and commercial importance. Elsevier/North-Holland, Amsterdam, p 13–21
Tomizawa JI, and Itoh T (1981) Plasmid Col E1 incompatibility determined by interaction of RNAI with primer transcript. Proc Natl Acad Sci USA 78:6096–6100
Veltkamp E, Barendsen W, Nijkamp HJJ (1974) Influence of protein and ribonucleic acid synthesis on the replication of the bacteriocinogenic factor Clo DF13 in Escherichia coli cells and minicells. J Bacteriol 118:165–174
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Communicated by D. Sherratt
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Hakkaart, M.J.J., Wesseling, J.G., Veltkamp, E. et al. Maintenance of the bacteriocinogenic plasmid clo DF13 in Escherichia coli cells. Mol Gen Genet 186, 531–539 (1982). https://doi.org/10.1007/BF00337961
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DOI: https://doi.org/10.1007/BF00337961